hrvatski

Assessment of Dental Implant Genotoxicity in Gingival Cells

Although titanium (Ti) implants are considered as bioinert, it is known that they can undergo corrosion and wear. The aim of this in vivo study was to evaluate genotoxic potential from two different implant systems in gingival epithelial cells. Exfoliated gingival cells were three times taken from 78 participants. DNA damages were analyzed using the micronucleus test before implant placement (T0), 90 days after dental implant placement i.e. immediately before gingiva former placement (T1), and 21 days after gingiva former placement (T2). Both implant systems, Ankylos and Dentium, showed a significant increase only in the number of binuclei (T0 vs T1 ; p = 0.011 and p ≤ 0.001). Furthermore, compared to the baseline values, after placing the gingiva formers (T0 vs T2), subjects had a significant increase in the number of micronucleus cells (p = 0.007 and p = 0.002) and binuclear cells (p = 0.006 and p ≤ 0.001). Based on the results, it can be concluded that there is no Ti implant-dependent cytotoxic or genotoxic effect in the gingival epithelial cells. The slightly increased cytogenetic damages cannot be labelled as biologically relevant.

dental implants ; genotoxicity ; gingival cells

nije evidentirano

engleski

Assessment of Dental Implant Genotoxicity in Gingival Cells

Although titanium (Ti) implants are considered as bioinert, it is known that they can undergo corrosion and wear. The aim of this in vivo study was to evaluate genotoxic potential from two different implant systems in gingival epithelial cells. Exfoliated gingival cells were three times taken from 78 participants. DNA damages were analyzed using the micronucleus test before implant placement (T0), 90 days after dental implant placement i.e. immediately before gingiva former placement (T1), and 21 days after gingiva former placement (T2). Both implant systems, Ankylos and Dentium, showed a significant increase only in the number of binuclei (T0 vs T1 ; p = 0.011 and p ≤ 0.001). Furthermore, compared to the baseline values, after placing the gingiva formers (T0 vs T2), subjects had a significant increase in the number of micronucleus cells (p = 0.007 and p = 0.002) and binuclear cells (p = 0.006 and p ≤ 0.001). Based on the results, it can be concluded that there is no Ti implant-dependent cytotoxic or genotoxic effect in the gingival epithelial cells. The slightly increased cytogenetic damages cannot be labelled as biologically relevant.

dental implants ; genotoxicity ; gingival cells

nije evidentirano