engleski

Measurement of the biomolecular interaction between plant lectin and peptidoglycan functionalized self-assembled hybrid bilayers by a quartz crystal microbalance

Peptidoglycan is the major component of bacterial cell walls which is recognized by the innate immune system through a series of pattern recognition receptors (PRR), which play a key role in first-line defense of the body. Lectins, naturally occurring carbohydrate- binding proteins, are involved in numerous biological processes and some of them act as PRR and bind significantly to PGN. In this study we were primarily interested to test interaction of peptidoglycan monomer (PGM), disaccharide pentapeptide isolated from B. divaricatum with model plant lectins, wheat germ agglutinin (WGA), by quartz crystal microbalance (QCM) method. In order to study interactions of PGM with lectins, lipophilic derivative, PGM-oleyl was synthesized and was used for preparation of the self-assembled hybrid bilayer membrane (HBM). It was demonstrated that PGM was effectively recognized by WGA and that strength of interactions depend on amount of PGM-oleil used for HBM preparation. The association konstant for the binding of WGA to PGM functionalized hybrid bilayers was determined. The results showed that the established QCM method for measurement of molecular recognition between lectin and peptidoglycan functionalized HBM could be successfully employ in analyses of lectin-carbohydrate interactions, such as specificity, affinity and kinetics.

peptidoglycan ; lectin ; quartz crystal microbalance ; hybrid bilayer membrane ; lectin-carbohydrate interaction

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