Self-assembled Hybrid Bilayer Membrane Functionalized by Bacterial Peptidoglycan as Biosensor for Lectin Recognition (CROSBI ID 685469)
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Podaci o odgovornosti
Štimac, Adela ; Frkanec, Leo ; Frkanec, Ruža
engleski
Self-assembled Hybrid Bilayer Membrane Functionalized by Bacterial Peptidoglycan as Biosensor for Lectin Recognition
Peptidoglycan is the major component of bacterial cell walls which is recognized by the innate immune system through a series of pattern recognition receptors (PRR), which play a key role in first-line defense of the body. Lectins, naturally occurring carbohydrate-binding proteins, are involved in numerous biological processes and some of them act as PRR and bind significantly to PGN. In this study we were primarily interested to test interaction of peptidoglycan monomer (GlcNAc- MurNac-LAla-D-isoGln-mesoDAP(εNH2)-DAla-D-Ala, PGM), disaccharide pentapeptide isolated from B. divaricatum with model plant lectin, wheat germ agglutinin (WGA), by quartz crystal microbalance (QCM) method (Figure 1). WGA has a high binding affinity for N-acetyl-glucosamine (GlcNAc), one of the essential components of PG glycan strands. In order to study interactions of PGM with lectins, lipophilic derivative, PGM-oleyl was synthesized and was used for preparation of the self-assembled hybrid bilayer membrane (HBM). The results showed that PGM was effectively recognized by WGA and that strength of interactions depend on amount of PGM-oleil used for HBM preparation. The association constant for the binding of WGA to PGM functionalized hybrid bilayers was determined. It was demonstrated that the use of functionalized self-assembled bilayer as a biosensing platform has the potential to become a powerful tool for the study of other glycans by using a greater variety of lectins.
peptidoglycan ; lectin ; quartz crystal microbalance ; hybrid bilayer membrane ; lectin-carbohydrate interaction
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Podaci o prilogu
25-25.
2019.
objavljeno
Podaci o matičnoj publikaciji
III. simpozij supramolekulske kemije, Knjiga sažetaka
Frkanec, Leo ; Namjesnik, Danijel ; Tomišić, Vladislav
Zagreb: Institut Ruđer Bošković
978-953-7941-27-7
Podaci o skupu
3. simpozij supramolekulske kemije (Supramolecular Chemistry 2019)
poster
03.12.2019-03.12.2019
Zagreb, Hrvatska
Povezanost rada
Kemija, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)