engleski

Significant changes of plasma N-glycome in patients with PTSD

Introduction: Posttraumatic stress disorder (PTSD) is trauma-related disorder that represents heavy socio-economic burden due to its complex and still unknown etiology, lack of validated biomarkers, and numerous comorbidities. Protein glycosylation affects majority of proteins present in blood plasma and can introduce changes in protein function and selectivity, therefore resulting in alternate cell signalling and possibly contributing to pathogenesis of different diseases as well as premature aging. Aim: The aim of this study was to examine N- glycome differences in blood plasma of PTSD and control subjects and to replicate the results in an independent cohort. Methods: All participants were unrelated males of Croatian origin. Plasma glycosylation was determined using ultra-high performance liquid chromatography resulting in 39 N-glycan peaks. The difference in N-glycan distribution between PTSD subjects and controls were evaluated with Mann-Whitney test and was corrected for the effect of age and multiple testing. Results: Out of 19 altered N-glycans in the first cohort, 6 N-glycans were replicated in both cohorts: 4 N-glycan were significantly higher, and 2 N-glycan were significantly lower in PTSD subjects compared to controls. These glycans were also following the same pattern of distribution in inflammatory processes and in older age. Conclusion: N-glycome of the individual is considered an adaptive answer to the environment. Specific changes of N-glycans in patients with PTSD, which were also observed in different pathophysiological states, suggest the role of N-glycans in PTSD pathogenesis. This research was supported by the Croatian Science Foundation, project No. IP-2014-09- 4289.

PTSD ; N-glycan ; biomarkers ; males

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