engleski

Genetic and glycomic interaction in posttraumatic stress disorder

Introduction Detection of specific and validated biomarkers of posttraumatic stress disorder (PTSD) is hindered by complexity of PTSD symptoms. Glycosylation is post-translational modification that affects majority of proteins and can radically change their biological properties. Process of sugar binding is not genetically determined, however, there are several genes that encode for the enzymes involved in metabolism and assembly of glycans, that could potentially contribute to the overall glycosylation pattern. Some of the most prominent single nucleotide polymorphisms (SNPs) are rs11621121, rs7953249, rs1257220 and rs3760776. The aim of this study was to determine the role of selected SNPs in patients with PTSD and controls. Materials and methods Plasma N-glycome was determined using ultra- high performance liquid chromatography in 233 male war veterans with PTSD and age- and sex- matched healthy control. Genotyping was done using real-time PCR. The differences in frequency and glycosylation pattern between different genotypes were calculated using chi- square test and ANOVA, respectively. Experiment was replicated in additional cohort consisted of 310 war veterans and controls. Results SNPs rs11621121, rs7953249, rs1257220 were differentially distributed between PTSD and control subjects, although alternations in glycosylation were not replicated in both cohorts. However, 5 plasma glycans were significantly different in both cohorts depending on rs3760776 genotype. Conclusions Our study showed that glycosylation of plasma proteins, in addition to several tested genes involved in this process, could have a role in development of PTSD and could represent a potential biomarker for PTSD. This research was supported by the Croatian Science Foundation, (IP-2014-09-4289).

PTSD ; polymorphism, N-glycan ; biomarkers

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