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Pregled bibliografske jedinice broj: 1037981

Pro-inflammatory effects of extracellular Hsp70 on NCI-H292 human bronchial epithelial cell line


Hulina-Tomašković, Andrea; Grdić Rajković, Marija; Jelić, Dubravko; Bosnar, Martina; Sladoljev, Lucija; Žanić Grubišić, Tihana; Rumora, Lada
Pro-inflammatory effects of extracellular Hsp70 on NCI-H292 human bronchial epithelial cell line // International journal of experimental pathology, 100 (2019), 5-6; 320-329 doi:10.1111/iep.12335 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 1037981 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Pro-inflammatory effects of extracellular Hsp70 on NCI-H292 human bronchial epithelial cell line

Autori
Hulina-Tomašković, Andrea ; Grdić Rajković, Marija ; Jelić, Dubravko ; Bosnar, Martina ; Sladoljev, Lucija ; Žanić Grubišić, Tihana ; Rumora, Lada

Izvornik
International journal of experimental pathology (0959-9673) 100 (2019), 5-6; 320-329

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
extracellular Hsp70 ; LPS ; LTA ; COPD ; NCI-H292 cells

Sažetak
Extracellular Hsp70 (eHsp70) exerts its biological actions via Toll-like receptors 2 and 4, and is increased in sera of chronic obstructive pulmonary disease (COPD) patients. The aim of this study was to explore the pro-inflammatory effects and cytotoxicity of eHsp70 alone and in combination with bacterial components lipoteichoic acid (LTA) and lipopolysaccharide (LPS) on NCI-H292 airway epithelial cells. NCI-H292 cells were treated with recombinant human Hsp70 protein (rhHsp70), LPS, LTA, and their combinations for 4, 12, 24, and 48 h. IL-6, IL-8 and TNF-α levels were measured by ELISA method. Cell viability was determined by MTS, and caspases-3/7, -8 and -9 assays. rhHsp70 induced secretion of IL-6 and IL-8 in a concentration- and time-dependent manner, with the highest secretion at 24 h. rhHsp70 combined with LTA had antagonistic, and with LPS synergistic effect on IL-6 secretion, while the interactions between rhHsp70 and LPS or LTA on IL-8 were synergistic. TNF-α was not detected in applied conditions. rhHsp70, LPS or LTA did not affect cell viability, and rhHsp70 even suppressed caspases-3/7 activities. We suggest that pro-inflammatory effects of eHsp70, together with other damaging molecules and/or COPD risk factors, might contribute to the aggravation of chronic inflammation in human bronchial epithel.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Farmacija



POVEZANOST RADA


Projekti:
IP-2014-09-1247 - Uloga stresnog proteina Hsp70 u imunosno-upalnom odgovoru kod kronične opstrukcijske plućne bolesti (Hsp70COPD) (HRZZ - 2014-09) ( POIROT)

Ustanove:
Farmaceutsko-biokemijski fakultet, Zagreb,
Fidelta d.o.o.

doi

Citiraj ovu publikaciju

Hulina-Tomašković, Andrea; Grdić Rajković, Marija; Jelić, Dubravko; Bosnar, Martina; Sladoljev, Lucija; Žanić Grubišić, Tihana; Rumora, Lada
Pro-inflammatory effects of extracellular Hsp70 on NCI-H292 human bronchial epithelial cell line // International journal of experimental pathology, 100 (2019), 5-6; 320-329 doi:10.1111/iep.12335 (međunarodna recenzija, članak, znanstveni)
Hulina-Tomašković, A., Grdić Rajković, M., Jelić, D., Bosnar, M., Sladoljev, L., Žanić Grubišić, T. & Rumora, L. (2019) Pro-inflammatory effects of extracellular Hsp70 on NCI-H292 human bronchial epithelial cell line. International journal of experimental pathology, 100 (5-6), 320-329 doi:10.1111/iep.12335.
@article{article, year = {2019}, pages = {320-329}, DOI = {10.1111/iep.12335}, keywords = {extracellular Hsp70, LPS, LTA, COPD, NCI-H292 cells}, journal = {International journal of experimental pathology}, doi = {10.1111/iep.12335}, volume = {100}, number = {5-6}, issn = {0959-9673}, title = {Pro-inflammatory effects of extracellular Hsp70 on NCI-H292 human bronchial epithelial cell line}, keyword = {extracellular Hsp70, LPS, LTA, COPD, NCI-H292 cells} }
@article{article, year = {2019}, pages = {320-329}, DOI = {10.1111/iep.12335}, keywords = {extracellular Hsp70, LPS, LTA, COPD, NCI-H292 cells}, journal = {International journal of experimental pathology}, doi = {10.1111/iep.12335}, volume = {100}, number = {5-6}, issn = {0959-9673}, title = {Pro-inflammatory effects of extracellular Hsp70 on NCI-H292 human bronchial epithelial cell line}, keyword = {extracellular Hsp70, LPS, LTA, COPD, NCI-H292 cells} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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