engleski

Pro-inflammatory effects of extracellular Hsp70 on NCI-H292 human bronchial epithelial cell line

Extracellular Hsp70 (eHsp70) exerts its biological actions via Toll-like receptors 2 and 4, and is increased in sera of chronic obstructive pulmonary disease (COPD) patients. The aim of this study was to explore the pro-inflammatory effects and cytotoxicity of eHsp70 alone and in combination with bacterial components lipoteichoic acid (LTA) and lipopolysaccharide (LPS) on NCI-H292 airway epithelial cells. NCI-H292 cells were treated with recombinant human Hsp70 protein (rhHsp70), LPS, LTA, and their combinations for 4, 12, 24, and 48 h. IL-6, IL-8 and TNF-α levels were measured by ELISA method. Cell viability was determined by MTS, and caspases-3/7, -8 and -9 assays. rhHsp70 induced secretion of IL-6 and IL-8 in a concentration- and time-dependent manner, with the highest secretion at 24 h. rhHsp70 combined with LTA had antagonistic, and with LPS synergistic effect on IL-6 secretion, while the interactions between rhHsp70 and LPS or LTA on IL-8 were synergistic. TNF-α was not detected in applied conditions. rhHsp70, LPS or LTA did not affect cell viability, and rhHsp70 even suppressed caspases-3/7 activities. We suggest that pro-inflammatory effects of eHsp70, together with other damaging molecules and/or COPD risk factors, might contribute to the aggravation of chronic inflammation in human bronchial epithel.

extracellular Hsp70 ; LPS ; LTA ; COPD ; NCI-H292 cells

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