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Pregled bibliografske jedinice broj: 1037978

Effects of extracellular Hsp70 and cigarette smoke on differentiated THP-1 cells and human monocyte-derived macrophages


Hulina-Tomašković, Andrea; Somborac-Bačura, Anita; Grdić Rajković, Marija; Bosnar, Martina; Samaržija, Miroslav; Rumora, Lada
Effects of extracellular Hsp70 and cigarette smoke on differentiated THP-1 cells and human monocyte-derived macrophages // Molecular immunology, 111 (2019), 53-63 doi:10.1016/j.molimm.2019.04.002 (međunarodna recenzija, članak, znanstveni)


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Naslov
Effects of extracellular Hsp70 and cigarette smoke on differentiated THP-1 cells and human monocyte-derived macrophages

Autori
Hulina-Tomašković, Andrea ; Somborac-Bačura, Anita ; Grdić Rajković, Marija ; Bosnar, Martina ; Samaržija, Miroslav ; Rumora, Lada

Izvornik
Molecular immunology (0161-5890) 111 (2019); 53-63

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
cigarette smoke, eHsp70, monocyte-derived macrophages, THP-1

Sažetak
Extracellular Hsp70 (eHsp70) can act as pro-inflammatory mediator and is elevated in blood of chronic obstructive pulmonary disease (COPD) patients. Most of those patients are smokers, and it was suggested previously that cigarette smoke might induce Hsp70 secretion from the circulating cells. Therefore, we aimed to explore inflammation-associated effects of cigarette smoke extract (CSE) and its combinations with eHsp70 in monocyte-derived macrophages (MDMs) and THP-1 cell line, used as systemic component models of COPD We hypothesized that eHsp70 induces inflammation, but that it can also modulate cigarette smoke extract (CSE)-stimulated inflammatory responses. We assessed IL-8 secretion, TLR2, TLR4 and Hsp70 expressions, MAPKs and NF-κB activation, and cytotoxicity after treating the cells with CSE (2.5 and 5%) and its combinations with low-endotoxin recombinant human (rh) Hsp70, used to mimic eHsp70 effects. CSE induced IL-8 secretion from both cell types, but its combinations with rhHsp70 increased IL-8 release compared to CSE alone only from MDMs. In THP-1, combinations of rhHsp70 with 2.5% CSE induced TLR2 and TLR4 mRNA, while 5% CSE decreased TLR2 expression. In MDMs, CSE alone attenuated TLR2, while rhHsp70 increased TLR2 and lowered TLR4 gene expression. Hsp70 mRNA expression was suppressed in THP-1 with rhHsp70 and CSE ; however, the same treatments increased its level in MDMs. CSE had cytotoxic effect only on MDMs, but cytotoxicity was reduced in co-treatments with rhHsp70, which also triggered apoptosis. CSE and rhHsp70 activated p38 and JNK, while ERK was activated only by rhHsp70 in MDMs. In THP-1, 2.5% CSE activated ERK, and 5% CSE activated p38. Inhibition of NF-κB and JNK in MDMs, and ERK and JNK in THP-1 cells, attenuated IL-8 release after rhHsp70 treatment. In conclusion, rhHsp70 provoked pro-inflammatory effects and could also modulate inflammatory response to CSE on protein and gene expression levels in THP-1 cells and MDMs, which suggests that eHsp70 might be implicated in systemic inflammation induced by cigarette smoke.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Farmacija



POVEZANOST RADA


Projekti:
HRZZ-IP-2014-09-1247 - Uloga stresnog proteina Hsp70 u imunosno-upalnom odgovoru kod kronične opstrukcijske plućne bolesti (Hsp70COPD) (HRZZ - 2014-09) ( POIROT)

Ustanove:
Farmaceutsko-biokemijski fakultet, Zagreb,
Klinika za plućne bolesti "Jordanovac",
Klinički bolnički centar Zagreb,
Fidelta d.o.o.

doi

Citiraj ovu publikaciju

Hulina-Tomašković, Andrea; Somborac-Bačura, Anita; Grdić Rajković, Marija; Bosnar, Martina; Samaržija, Miroslav; Rumora, Lada
Effects of extracellular Hsp70 and cigarette smoke on differentiated THP-1 cells and human monocyte-derived macrophages // Molecular immunology, 111 (2019), 53-63 doi:10.1016/j.molimm.2019.04.002 (međunarodna recenzija, članak, znanstveni)
Hulina-Tomašković, A., Somborac-Bačura, A., Grdić Rajković, M., Bosnar, M., Samaržija, M. & Rumora, L. (2019) Effects of extracellular Hsp70 and cigarette smoke on differentiated THP-1 cells and human monocyte-derived macrophages. Molecular immunology, 111, 53-63 doi:10.1016/j.molimm.2019.04.002.
@article{article, year = {2019}, pages = {53-63}, DOI = {10.1016/j.molimm.2019.04.002}, keywords = {cigarette smoke, eHsp70, monocyte-derived macrophages, THP-1}, journal = {Molecular immunology}, doi = {10.1016/j.molimm.2019.04.002}, volume = {111}, issn = {0161-5890}, title = {Effects of extracellular Hsp70 and cigarette smoke on differentiated THP-1 cells and human monocyte-derived macrophages}, keyword = {cigarette smoke, eHsp70, monocyte-derived macrophages, THP-1} }
@article{article, year = {2019}, pages = {53-63}, DOI = {10.1016/j.molimm.2019.04.002}, keywords = {cigarette smoke, eHsp70, monocyte-derived macrophages, THP-1}, journal = {Molecular immunology}, doi = {10.1016/j.molimm.2019.04.002}, volume = {111}, issn = {0161-5890}, title = {Effects of extracellular Hsp70 and cigarette smoke on differentiated THP-1 cells and human monocyte-derived macrophages}, keyword = {cigarette smoke, eHsp70, monocyte-derived macrophages, THP-1} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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