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Sterilization of nutrient media for in vitro cultivation of plants

Sterilization of nutrient media for in vitro propagation of plants is first step towards successful plant production. Most commonly used method for medium sterilization is autoclaving. Although it is a proven method for successful sterilization, if all regulations are respected, it is an expensive and time- consuming method as well. Alternatives to autoclaving for achieving a sterile nutrient medium are filtration, microwave sterilization and chemical sterilization.

nutrient medium ; sterilization ; autoclave ; microwave ; filtartion

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