engleski

The use of a biofixators for the removal of AFM1 from milk

The EU RASFF (Rapid Alert System for Food and Feed) system observed the significant presence of mycotoxins in food and feed. It is considered that 25% from the total quantity of produced food in the world, mainly of plant origin, are contaminated with mycotoxins. Due to their toxicity, it is important to control their presence and quantity in foods. Aflatoxin M1 is formed as the product of biological conversion of aflatoxin B1 in the mammary glands of mammals fed with fodder containing aflatoxin B group. Therefore, mycotoxins are entering in human food chain, not only through cereals and cereal products, but also through the meat, eggs, milk and dairy products. It is important to find accurate, reliable and efficient strategy for removal of inherent mycotoxins in food and/or fodder supplements. In this study, removal of mycotoxins with microbial cultures and their cellular components are imposed as an alternative to existing physical and chemical methods of detoxification. The project aims to explore the application of lactic acid bacteria (LAB) isolated from traditional dairy products and yeast cell components (β-glucan), and possible removal of resulting complex with mycotoxin from the milk. The research included quantification of the amount of AFM1 in milk samples using HPLC-FLD and LC-MS/MS followed by application of different concentrations of live and dead cells of LAB, lyophilized live and dead cells of LAB, as well as application of β- glucan (commercial and isolated from yeast cells). Formed biofixator - AFM1 complex was removed using the membrane filtration. Quality of milk regarding the quantity of macronutrients (fat, fatty acid composition, protein and carbohydrates and lactose) were determined.

AFM1, lactobacillus, biofixator, milk

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