engleski

REGIONAL HLA-A~B~C~DRB1 HAPLOTYPE DIFFERENCES IN CROATIA

Population studies focused on HLA genes, due to their high allelic diversity, are of great importance for tracking changes in evolutionary history of different populations, identification of conserved combinations of different alleles and linkage disequilibrium among them. The aim of this study was to compare the distribution of three most common HLA 344 ABSTRACTS haplotypes in the general Croatian population with their regional distribution in a total of 4792 volunteer unrelated donors from ten different recruitment cities across Croatia (N=243 to N=1071). The capital, Zagreb, was excluded from regional distribution analysis. All individuals were tested for HLA-A, -B, -C, and -DRB1 alleles by PCR-SSO and PCR-SSP methods. The previously published results were used for general population haplotype frequencies. The most common haplotype in the general population, as well as in all ten cities was HLA-A*01:01~B*08:01~ C*07:01~DRB1*03:01. Its regional frequency distribution ranged from 3.7% to 7.0%, and did not show differences in regional distribution nor when compared with general population (4.2%). HLA- A*02:01~B*18:01~C*07:01~DRB1* 11:04, as the second ranked haplotype in general population (1.5%), showed regional distribution frequency differences ranging from 0.8% to 3.2%, with a haplotype position ranking from 2 to 16. The observed differences did not reach statistical significance. The third most common haplotype in general population HLA-A*03:01~B*07:02~C*07:02~ DRB1*15:01 (1.2%) showed high statistically significant regional distribution differences, with a frequency range from 0.2% to 3.0%. It was ranked as second or third most common haplotype in a total of four cities, in four cities it had haplotype position ranking from 5-10, in one city it was present only once while in one city it was not detected at all. This type of study would be useful for optimizing donor recruitment strategies and registry planning.

HLA, haplotypes, Croatian population

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