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DETERMINATION OF THE ANTIVIRAL POTENTIAL OF BENZIMIDAZOLIC COMPOUNDS IN FRONT OF ZIKA VIRUS

Background: Benzimidazoles are aromatic heterocyclic organic compounds which consist of the melting of benzene and imidazole. These compounds have several applications in the pharmacological area with antineoplastic, anti- inflammatory, analgesic, anthelmintic, antiparasitic and antimicrobial action. Therefore, taking into account the great diversity of applications of benzimidazoles, we evaluated the antiviral potential of NB2 and NB5 compounds against the Zika virus (ZIKV). Methods: Vero cells, susceptible to ZIKV, were cultured in DMEM supplemented with 10% fetal bovine serum and maintained in a humid atmosphere at 37°C with 5% CO2. Subsequently, the least squares regression method was used to obtain the cytotoxic concentration for 50% of the culture (CC50) through the tetrazolium salt reduction assay MTT, which determines the mitochondrial functionality. These values were used to define the test concentrations for the antiviral analysis. For this, 2.5x105 cells/well were inoculated into 24- well plates, and the viral suspensions were adjusted to 100 PFU/well. Infected cells were exposed for 72 hours at concentrations of 0.3 to 10 μM of compounds NB2 and NB5. The tests were performed using the plaque assay method in medium with 2% CMC and 0.4% crystal violet staining. Results: The results obtained in the determination of CC50 indicated concentration- dependent toxicity profile for both compounds, with values 15.73 μM and 0.85 μM for NB2 and NB5, respectively. In the antiviral analysis the highest reduction was 43.8% for NB2 and 41.9% for NB5, both at concentration of 0.6 μM. However, a cytotoxic effect was observed in the plaque assay for NB5 at concentrations higher than 2.5 μM. Conclusions: It may be concluded that compounds NB2 and NB5 showed a weak antiviral potential against ZIKV. However, further trials using other methodologies are required to confirm these results.

Cell culture, Pharmacological activity, ZIKV, Benzimidazoles

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