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Pregled bibliografske jedinice broj: 1032930

Simultaneous determination of aflatoxins and ochratoxin A in traditional dry-fermented sausages using LC-MS/MS method


Pleadin, Jelka; Lešić, Tina; Vulić, Ana; Kudumija, Nina; Vahčić, Nada
Simultaneous determination of aflatoxins and ochratoxin A in traditional dry-fermented sausages using LC-MS/MS method // Book of Abstracts of 33rd EFFoST International Conference 2019: Sustainable Food Systems - Performing by Connecting
Rotterdam, Nizozemska, 2019. str. P2204-P2204 (poster, međunarodna recenzija, sažetak, znanstveni)


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Naslov
Simultaneous determination of aflatoxins and ochratoxin A in traditional dry-fermented sausages using LC-MS/MS method

Autori
Pleadin, Jelka ; Lešić, Tina ; Vulić, Ana ; Kudumija, Nina ; Vahčić, Nada

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts of 33rd EFFoST International Conference 2019: Sustainable Food Systems - Performing by Connecting / - , 2019, P2204-P2204

Skup
33rd EFFoST International Conference 2019: Sustainable Food Systems - Performing by Connecting

Mjesto i datum
Rotterdam, Nizozemska, 12.-14.11.2019

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Aflatoxins ; Ochratoxin A ; LC-MS/MS ; Traditional meat products

Sažetak
Standardization of quality and safety of traditional meat products (TMPs) require research of potential sources of contamination. During ripening, TMPs are overgrown with moulds, some of which can produce mycotoxins as secondary metabolites harmful to human health. Mycotoxins can also enter TMPs via contaminated feed farm animals are fed on (carry-over effect) or via spices used in the production. Mycotoxins of the highest significance for public health are aflatoxin B1 and ochratoxin A, for which major toxic effects in humans and animals are proven. In this study, analytical method for the determination of five mycotoxins: aflatoxins B1, B2, G1 and G2 (AFB1, AFB2, AFG1 and AFG2) and ochratoxin A (OTA), underpinned by simultaneous extraction and clean-up using immunoaffinity columns, followed by liquid chromatography coupled with tandem mass spectrometry (LC–MS/MS) using a triple quadrupole-mass spectrometer, was developed. Validation procedures for aflatoxins and OTA showed the mean recovery rates of 76.92% and 85.47%, and coefficients of variation (CV) of 0.11% and 0.16%, respectively. The limits of detection (LODs) for all four aflatoxins and OTA were 0.10 μg/kg and 0.25 μg/kg, respectively. This analytical method was used to analyse 36 samples of traditional dry- fermented sausages sampled in 2019 from different Croatian regions. Whereas the presence of aflatoxins was not detected, OTA was detected in 14% of samples in concentrations ranging from 0.25 to 0.48 μg/kg. Given that legislation has not laid down the maximum levels (MLs) for mycotoxins in meat products yet, and taking into account that data on their occurrence is scarce, highly sensitive methods should be used in order to gain insight into mycotoxin levels in TMPs produced using different technologies under different conditions, so as to ensure meat product safety and protect human health.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija, Prehrambena tehnologija



POVEZANOST RADA


Projekt / tema
HRZZ-IP-2018-01-9017 - Mikotoksini u hrvatskim tradicionalnim mesnim proizvodima: molekularna identifikacija plijesni producenata i procjena izloženosti potrošača (TMPmouldRISK) (Pleadin, Jelka, HRZZ - 2018-01 )

Ustanove
Hrvatski veterinarski institut, Zagreb,
Prehrambeno-biotehnološki fakultet, Zagreb

Profili:

Avatar Url Ana Vulić (autor)

Avatar Url Nada Vahčić (autor)

Avatar Url Jelka Pleadin (autor)

Avatar Url Nina Kudumija (autor)

Avatar Url Tina Lešić (autor)

Citiraj ovu publikaciju

Pleadin, Jelka; Lešić, Tina; Vulić, Ana; Kudumija, Nina; Vahčić, Nada
Simultaneous determination of aflatoxins and ochratoxin A in traditional dry-fermented sausages using LC-MS/MS method // Book of Abstracts of 33rd EFFoST International Conference 2019: Sustainable Food Systems - Performing by Connecting
Rotterdam, Nizozemska, 2019. str. P2204-P2204 (poster, međunarodna recenzija, sažetak, znanstveni)
Pleadin, J., Lešić, T., Vulić, A., Kudumija, N. & Vahčić, N. (2019) Simultaneous determination of aflatoxins and ochratoxin A in traditional dry-fermented sausages using LC-MS/MS method. U: Book of Abstracts of 33rd EFFoST International Conference 2019: Sustainable Food Systems - Performing by Connecting.
@article{article, year = {2019}, pages = {P2204-P2204}, keywords = {Aflatoxins, Ochratoxin A, LC-MS/MS, Traditional meat products}, title = {Simultaneous determination of aflatoxins and ochratoxin A in traditional dry-fermented sausages using LC-MS/MS method}, keyword = {Aflatoxins, Ochratoxin A, LC-MS/MS, Traditional meat products}, publisherplace = {Rotterdam, Nizozemska} }




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