engleski

Biomolecular interactions of plant lectin and self-assembled hybrid bilayers modified with bacterial peptidoglycan

Peptidoglycan is the major component of bacterial cell walls which is recognized by the innate immune system through a series of pattern recognition receptors (PRR), which play a key role in first-line defense of the body. [1] Lectins, naturally occurring carbohydrate- binding proteins, are involved in numerous biological processes and some of them act as PRR and bind significantly to PGN.[2] Therefore, lectins are subject to extensive studies in the fields of infectious diseases and cancer research as potential drug targets or therapeutic agents. In this study we were primarily interested to test interaction of peptidoglycan monomer (PGM), [3] disaccharide pentapeptide isolated from B. divaricatum with model plant lectins by quartz crystal microbalance (QCM) method. In order to study interactions of PGM with lectins, lipophilic derivative, PGM-oleyl was synthesized and was used for preparation of the self-assembled hybrid bilayer membrane (HBM). It was demonstrated that PGM was effectively recognized by wheat germ agglutinin (WGA) and that strength of interactions depend on amount of PGM-oleil used for HBM preparation. Since the peptidoglycan recognition by PRRs involves moderate- to high-affinity interactions with the carbohydrate moiety as well as the peptide moiety of peptidoglycan, the established method could be successfully employ in analyses of lectin-carbohydrate interactions, such as specificity, affinity and kinetics.

peptidoglycan ; lectin ; quartz crystal microbalance ; hybrid bilayer membrane ; lectin-carbohydrate interaction

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