engleski

Comparison of two in vitro digestion methods for evaluation of bioaccesibility of minerals in fresh wheatgrass juice

Minerals are essential nutrients that support a wide variety of bodily functions. Food is the main source of minerals for humans, and some foods are better sources of minerals than others. The aim of this research was to compare two static in vitro digestion methods (Kiers et al. (2000) and Minekus et al. (2014)) for determination of bioaccessibility of K, Ca, Mg, Mn, Fe, and Zn in fresh wheatgrass juice. The experiment was carried out on a 25 wheat cultivars, grown in controlled conditions for 12 days. Higher mean concentrations of Ca (24%), Mg (7 %), K (10%), and Mn (37%) were determined (ICP-OES technique) after simulation of in vitro digestion by Kiers et al. (2000), while higher Fe (38%) and Zn (65%) concentrations were determined after simulation of in vitro digestion by Minekus et al. (2014). Correlation of mineral concentrations between two methods was significant (p<0.001) for all elements, and varied between r=0.56 for Zn to r=0.92 for Fe. In conclusion, it is not clear which method is better, so further research on a larger sample and with more replicates should be carried out.

wheatgrass ; bioavailability ; biofortification ; CaCo-2

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