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Antioxidative properties of hydolates and their potentially protective effect on lipid peroxidation

Introduction Hydrolates, also known as a hydrosols or aromatic waters, are the byproducts in production of natural essential oils. After removing essential oil, the remaining 90% of the water is considered hydrolate. They are popularly used directly on the skin or as an aqueous phase in creams. Therefore, the aim of the study was to determine antioxidant capacity of different hydolates and their potentially protective effect on lipid peroxidation, using different in vitro models. Materials and methods Commercially available hydrolates from rose, immortelle, German chamomile, Roman chamomile, lavender, sage, and geranium were obtained for this study. Spectrophotometric (Trolox equivalent antioxidant capacity (TEAC)) and spectrofluorometric (Oxygen radical absorbance capacity (ORAC)) methods were used to determine total antioxidant capacity (AOX) of different hydolates. In order to investigate potentially protective effect of tested hydolates on lipid peroxidation, two hydolates with highest level of antioxidant capacity were applied with linolenic acid (LNA) and exposed to ultraviolet (UV) radiation, as a model of oxidative stress. Lipid peroxides were measured by thiobarbituric acid (TBA) assay and quantified by spectrophotometry. Results Rose hydrolate showed the highest AOX level measured by TEAC method, and lavender hydrolate showed the highest AOX level measured by ORAC method. Therefore, these two hydrolates were applied with LNA and exposed to UV. While lavender hydrolate showed mild antioxidant effect on lipid peroxidation, rose hydrolate induced an increase of lipid peroxides when compared to control. Conclusions Further studies are warranted to investigate antioxidant effect of hydolates ex vivo and in vivo after topical application.

hydolates ; antioxidant capacity ; lipid peroxidation ; UV radiation

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