Naslov
EFFECT OF DIPEPTIDYL-PEPTIDASE IV (DPP IV/CD26) DEFICIENCY ON CUTANEOUS WOUND HEALING PROCESS IN A STREPTOZOTOCIN-INDUCED DIABETES MODEL IN MICE

Autori
Batičić, Lara ; Detel, Dijana ; Pernjak Pugel, Ester ; Varljen, Jadranka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts - HDBMB2019 - Crossroads in Life Sciences / Katalinić, Maja ; Dulić, Morana ; Stuparević, Igor - Zagreb : Hrvatsko Društvo za Biotehnologiju, 2019, 59-59

ISBN
978-953-95551-7-5

Skup
Congress of the Croatian Society of Biochemistry and Molecular Biology "Crossroads in Life Sciences" (HDBMB2019)

Mjesto i datum
Lovran, Hrvatska, 25.09.2019. - 28.09.2019

Vrsta sudjelovanja
Pozvano predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
DPP IV/CD26, diabetes model, wound healing, angiogenesis

Sažetak
Dipeptidyl-peptidase IV or CD26 molecule (DPP IV/CD26) is widely known, besides the involvement in a broad range of physiological and pathological processes, for its role in regulation of glycaemia. DPP IV/CD26 inhibitors are currently available for the treatment of type 2 diabetes. Diabetes-related complications such as ulceration, infection, and gangrene are the leading causes of hospitalization of patients with diabetes mellitus. It has been proposed that DPP IV/CD26 inhibition accelerates healing of chronic diabetic ulcers, through induction of a histological pattern consistent with enhanced angiogenesis. However, in spite of its known enzymatic and immunomodulative functions as well involvement in maintaining the homeostasis of glucose, limited data is available regarding the role of DPP IV/CD26 in wound healing mechanisms. We hypothesized a significant role of DPP IV/CD26 in the process of cutaneous wound healing in hiperglicaemia. Our aim was to investigate the process of wound healing in conditions of CD26 deficiency in experimental hiperglicaemia in order to obtain more insights on the role of DPP IV/CD26 in cutaneous reparation and regeneration. A streptozotocin-model of diabetes was induced in CD26-deficient and wild-type mice. Diabetic mice were wounded on the dorsal region and sacrificed in scheduled time periods. Control skin and wound samples were subjected to pathohistological, histomorphometrical, immunohistochemical and immunochemical analyses while serum samples were analysed for DPP IV/CD26 activity and concentration of target angiogenic factors. The results of this study confirm the hypothesis that DPP IV/CD26 plays an important role in the regulation of blood glucose concentration and that its inactivation improves the state related with hiperglicaemia. Furthermore, the process of cutaneous wound healing is improved in conditions of DPP IV/CD26 deficiency with increased local expression of proangiogenic factors, confirming that inhibition of this molecule has beneficial effects on the wound healing process in hiperglicaemia. Based on these results, the significance of DPP IV/CD26 inhibition as a therapeutic option for the treatment of diabetes and its complications is proposed.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA