engleski

The red beetroot peel extract – characterization of bioactive composition and biological activity in vitro

The inedible part of the red beetroot-peel (RBP) is considered as an agro-industrial waste, athough it also could be a great source of valuable bioactive compounds. Therefore, the aim of this study was to investigate the utilization possibilities of RBP in the production of value added compounds through the characterization of bioactive composition and in vitro biological properties of its extract (RBPE). Total phenolic (15, 43±0, 34 mg GAE/g dw) and betacyanins (9, 80±0, 14 mg betanin/g dw) content, as well as antioxidant capacity using DPPH and ABTS assays (0, 0190±0, 00 and 0, 0561±0, 00 mmol Trolox/g dw, respectively) of RBPE was determined. The biological effect of RBPE on the human hepatic (HepG2), tongue (CAL27) and colon (Caco2) cancer cell lines was conducted. Cell survival was determined by Neutral red test and free radicals determination with 2’, 7’-dichlorofluorescein- diacetate method. The protective effect of RBPE against oxidative DNA damage was detected on supercoiled plasmid ΦX174 RF1. RBPE did not cause a cytotoxic effect on evaluated cell lines. On colon cancer cells it showed antioxidative properties but its higher concentration during a prolonged time of incubation showed a slight prooxidative effect on liver cells. Compared to RBPE without polysaccharides, extract with polysaccharides showed a higher protective effect against oxidative damage of DNA. Results confirmed the red beetroot peel as a great source of bioactive compounds which synergistic effect has an important contribution to the cellular macromolecules protection.

red beetroot peel ; cytotoxicity ; hepatic, tongue and colon cancer cell lines ; oxidative DNA damage ; antioxidans

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