engleski

Assessment of K2(B3O3F4OH) induced DNA damage in peripheral blood cultures

As one of the leading causes of mortality and morbidity, cancer is the focus of many researches aimed on the development of novel anticancer agents. Halogenated boroxine K2 (B3 O3 F4 OH), heterocyclic derivate of boronic acid, has been patented as a potential therapeutic for benign and malignant skin changes. Boron is known as important microelement in wound healing. Due to its bioactivity the interest for the development of boron containing compounds, including anti-cancer drugs, has significantly grown. Although various in vitro and in vivo analysis of K2 (B3 O3 F4 OH) genotoxicity were previously conducted, this study aimed to analyse DNA damage in cultures of whole peripheral blood from healthy volunteer using alkaline comet assay. Separate cultures for 1 and 24 hours treatments with 0.1, 0.2 and 0.4 mg/ml K2 (B3 O3 F4 OH) together with untreated controls were established. Duplicate slides (200 comets in total) per each treatment were analysed using Comet Assay IV software. Mean values of two tail intensity (TI) medians were compared among samples. Higher DNA damage was seen for 0.2 mg/ml, with significant difference revealed for 0.4 mg/ ml. These results correlate with previous findings of K2 (B3 O3 F4 OH) peripheral blood cultures treatment with chromosomal aberration and micronucleus assay. Results also confirmed 0.2 mg/ml as a limit dose for appearance of K2 (B3 O3 F4 OH) induced genotoxic events in vitro. This work was supported by CA15132 Cost Action, Ministry of Civil Affairs of B&H and Ministry of Education, Science and Youth of Sarajevo Canton. Hadzic M, et al.. Inhibitory effects of delphinidin and luteolin on genotoxicity induced by K2[B3O3F4OH] in human lymphocytes in vitro. Biologia. 2015 ; 70:553-558. Haveric S, et al. Effects of dipotassium trioxohydroxytetrafluorotriborate (K2[B3O3F4OH]) on genetic material and inhibition of cell division in human cell cultures. Drug Chem Toxicol. 2011 ; 34(3):250-254. Haveric S, et al. Genotoxicity Evaluation of Dipotassium-Trioxohydroxytetrafluorotriborate, K2(B3O3F4OH), in Human Lymphocyte Cultures and Mice Reticulocytes. Braz Arch Biol Techn. 2016 ; 59:e16160195.

Halogenated boroxine ; comet assay ; Lymphocytes ; DNA damage

nije evidentirano