engleski

THE ACTIVITY OF GARLIC EXTRACTS (ALLIUM SATIVUM) TO THE EPITHELIAL DAMAGE CAUSED BY SODIUM TAUROCHOLATE IN A CELL CULTURE MODEL OF ULCER DISEASE

Introduction: Peptic ulcer is a chronic disease affecting up to 10% of the world's population. A cellular model of ulcer disease can be established in the human gastric cell line (AGS) using bile salts such as sodium taurocholate (NaT). Hence, the aim was to establish a cell culture model of ulcer disease, measure the toxic effect of NaT and to determine possible inhibition of NaT caused oxidative stress by antioxidant treatment of garlic extracts. Materials and Methods: AGS cell line was used to develop the cell culture model of ulcer disease by NaT exposure. To prevent oxidative stress, AGS cells were co-treated with different concentrations of garlic extracts (GE). The extent of cytotoxicity and apoptosis was evaluated by cell counting and MTT, respectively. The extent of GE's gastroprotective effect was evaluated by expression of superoxide dismutase (SOD) by RT PCR. Results: Sodium taurocholate caused a significant reduction in the viability of AGS. It was observed that cells treated with NaT and co-treated with GE showed significant increase in viability, compared to the cells treated solely with NaT. SOD expression had shown positive correlation with antioxidant treatment. Conclusion: Garlic extracts co-treatment demonstrated gastroprotective effect on AGS cell culture model of ulcer disease. However, more experiments are needed to elucidate effect of garlic extracts in gastric ulcer disease.

garlic ; sodium taurocholate ; peptic ulcer

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