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Pregled bibliografske jedinice broj: 1030837

Optimization of a fast screening method for the assessment of low molecular weight thiols in human blood and plasma suitable for biomonitoring studies


Duka, Ivan; Gerić, Marko; Gajski, Goran; Friščić, Maja; Maleš, Željan; Domijan, Ana- Marija; Turčić, Petra
Optimization of a fast screening method for the assessment of low molecular weight thiols in human blood and plasma suitable for biomonitoring studies // Journal of Environmental Science and Health, Part A: Toxic/Hazardous Substances and Environmental Engineering, 55 (2020), 3; 275-280 doi:10.1080/10934529.2019.1687236 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 1030837 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Optimization of a fast screening method for the assessment of low molecular weight thiols in human blood and plasma suitable for biomonitoring studies

Autori
Duka, Ivan ; Gerić, Marko ; Gajski, Goran ; Friščić, Maja ; Maleš, Željan ; Domijan, Ana- Marija ; Turčić, Petra

Izvornik
Journal of Environmental Science and Health, Part A: Toxic/Hazardous Substances and Environmental Engineering (1093-4529) 55 (2020), 3; 275-280

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
low molecular weight thiols ; glutathione ; storage conditions ; antioxidant ; biomonitoring

Sažetak
An adequate level of low molecular weight thiols (LMW-SH, especially glutathione (GSH)) protects cellular macromolecules against toxic agents, and is used as a sensitive biomarker of exposure to toxic compounds. During sample collection, storage and preparation, non-enzymatic and enzymatic oxidation of LMW-SH can occur leading to analytical inaccuracy. The aim of this study was to optimize a fast and reliable screening method for the determination of LMW-SH, mainly GSH, in blood and plasma samples as well as to investigate the impact of storage conditions on the LMW-SH stability. Based on our results, the described spectrophotometric method allows fast and reliable determination of LMW-SH in blood and plasma samples. Results on incubation of samples at 37 °C imply that synthesis of LMW-SH (probably GSH) as well as dynamic interexchange among various thiols forms can be induced in blood cells in in vitro conditions. Importantly, the level of LMW-SH in blood and plasma stored at −20 °C was constant, indicating that they can be stored at −20 °C for at least 30 days. Therefore, the method is suitable for assessment of LMW-SH in long-term human biomonitoring as well as environmental field studies, especially those involving a large number of samples such as epidemiological studies.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija



POVEZANOST RADA


Ustanove:
Farmaceutsko-biokemijski fakultet, Zagreb,
Institut za medicinska istraživanja i medicinu rada, Zagreb

Citiraj ovu publikaciju

Duka, Ivan; Gerić, Marko; Gajski, Goran; Friščić, Maja; Maleš, Željan; Domijan, Ana- Marija; Turčić, Petra
Optimization of a fast screening method for the assessment of low molecular weight thiols in human blood and plasma suitable for biomonitoring studies // Journal of Environmental Science and Health, Part A: Toxic/Hazardous Substances and Environmental Engineering, 55 (2020), 3; 275-280 doi:10.1080/10934529.2019.1687236 (međunarodna recenzija, članak, znanstveni)
Duka, I., Gerić, M., Gajski, G., Friščić, M., Maleš, Ž., Domijan, A. & Turčić, P. (2020) Optimization of a fast screening method for the assessment of low molecular weight thiols in human blood and plasma suitable for biomonitoring studies. Journal of Environmental Science and Health, Part A: Toxic/Hazardous Substances and Environmental Engineering, 55 (3), 275-280 doi:10.1080/10934529.2019.1687236.
@article{article, year = {2020}, pages = {275-280}, DOI = {10.1080/10934529.2019.1687236}, keywords = {low molecular weight thiols, glutathione, storage conditions, antioxidant, biomonitoring}, journal = {Journal of Environmental Science and Health, Part A: Toxic/Hazardous Substances and Environmental Engineering}, doi = {10.1080/10934529.2019.1687236}, volume = {55}, number = {3}, issn = {1093-4529}, title = {Optimization of a fast screening method for the assessment of low molecular weight thiols in human blood and plasma suitable for biomonitoring studies}, keyword = {low molecular weight thiols, glutathione, storage conditions, antioxidant, biomonitoring} }
@article{article, year = {2020}, pages = {275-280}, DOI = {10.1080/10934529.2019.1687236}, keywords = {low molecular weight thiols, glutathione, storage conditions, antioxidant, biomonitoring}, journal = {Journal of Environmental Science and Health, Part A: Toxic/Hazardous Substances and Environmental Engineering}, doi = {10.1080/10934529.2019.1687236}, volume = {55}, number = {3}, issn = {1093-4529}, title = {Optimization of a fast screening method for the assessment of low molecular weight thiols in human blood and plasma suitable for biomonitoring studies}, keyword = {low molecular weight thiols, glutathione, storage conditions, antioxidant, biomonitoring} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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