engleski

GASTROPROTECTIVE EFFECT O OF GARLIC EXTRACTS (ALLIUM SATIVUM) ON AMELIORATING DAMAGE CAUSED BY SODIUM TAUROCHOLATE IN A CELL MODEL OF ULCER DISEASE

Abstract: Garlic (Allium sativum) is one of the oldest cultivated plants with significant dietary and medicinal role. It has been used as a spice, food, and herbal medicine for over 4000 years. Numerous studies have shown the gastroprotective effect of garlic extracts (GE). Therefore, the aim was to establish a cell culture model of ulcer disease using bile salts such as sodium taurocholate (NaT) and to determine inhibition of NaT caused oxidative stress by antioxidant treatment of GE. Human gastric cell line (AGS) was used as a cellular model of ulcer disease. Cells were co-treated with different concentrations of garlic extracts (GE). The cytotoxicity of NaT against AGS cells was determined by cell counting. The oxidative stress was evaluated by expression of superoxide dismutase (SOD) by RT PCR. NaT caused a significant reduction in the viability of AGS. It was demonstrated that cells treated with NaT and co-treated with GE showed significant increase in viability, compared to the cells treated solely with NaT. It has been demonstrated a positive correlation of SOD with antioxidant treatment. Garlic extracts co- treatment showed gastroprotective effect on AGS cell culture model of ulcer disease. Nevertheless, further experiments are needed to confirm protective role of garlic extracts in gastric ulcer disease.

garlic extracts, herbal medicine, ulcer disease, sodium taurocholate, AGS cell line

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