POTENTIAL OF CAFFEINE TREATMENT ON OXIDATIVE STRESS AND CRYSTALLIZATION INHIBITION IN CELL CULTURE MODEL OF OXALATE UROLITHIASIS (CROSBI ID 683263)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Ninčević, Vjera ; Kizivat, Tomislav ; Cindrić, Anita ; Omanović Kolarić, Tea ; Kuna, Lucija ; Smolić, Robert ; Bilić Ćurčić, Ines ; Smolić, Martina
engleski
POTENTIAL OF CAFFEINE TREATMENT ON OXIDATIVE STRESS AND CRYSTALLIZATION INHIBITION IN CELL CULTURE MODEL OF OXALATE UROLITHIASIS
Caffeine is a naturally occurring central nervous system stimulant and it is found in around sixty plant species. Most common sources are the beans of Coffea arabica and Coffea canephora. Recently it has been shown that caffeine possesses antioxidant properties as well. Further, urolithiasis is a disease defined by formation of solid deposits and most of the stones inside the urinary tract are predominantly composed of calcium oxalate monohydrate (COM) which can produce free radicals. The used cell culture as in vitro model of urolithiasis was the Lilly Laboratories Cell Porcine Kidney (LLC-PK type I). Oxidative stress was caused by exposing cells to COM crystals. Cells were treated with various concentrations of caffeine. Effects of caffeine on crystallization and oxidative stress was evaluated by COM crystallization assay and total glutathione assay, respectively. Caffeine treatment blocked the crystallization of COM in in vitro model of proximal kidney tubules urolithiasis. Total number of COM crystals was decreasing in correlation with increasing concentrations of caffeine up to 60% and with the duration of treatment with caffeine. However, concentrations of total glutathione altered up to 1, 8% only after caffeine treatment. Caffeine inhibits crystallization of COM, however its antioxidant potential in the cellular model of oxalate urolithiasis of proximal kidney tubules LLC-PK type I remains elusive.
caffeine ; calcium oxalate ; LLC-PK1 ; urolithiasis ; glutathione
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Podaci o prilogu
73-73.
2019.
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objavljeno
Podaci o matičnoj publikaciji
Knjiga sažetaka s 12. međunarodnog znanstveno- stručnog skupa HRANOM DO ZDRAVLJA
Podaci o skupu
12. međunarodni znanstveno-stručni skup: hranom do zdravlja = 12th International Scientific and Professional Conference: With food to health
poster
24.10.2019-25.10.2019
Osijek, Hrvatska; Tuzla, Bosna i Hercegovina