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Antimicrobial activity and immunomodulating potential of Lactobacillus plantarum M2 isolated from donkey milk

Traditionally donkey milk has been used as an alternative to human milk for infants or children mainly because of its similarities in nutrient composition, hypoallergenicity, immune modulation and antimicrobial activity. As a highly nutritious substrate it has a great potential for probiotic bacteria isolation. Beneficial health effects of probiotic bacteria used in functional foods and pharmaceutical products are based on the ability and capacity of these microorganisms in stimulation of host microbiota and immune system.Theaim of the study was to assess Lactobacillus plantarumM2’s probiotic potential through antimicrobial activity and immunomodulation potential. L. plantarumM2 was isolated from donkey milk and identified using biochemical and genetic tests. Antimicrobialactivity against several pathogens was determined using turbidimetric method. TNF-α production of LPS-stimulated human blood mononuclear cells (PBMC) was determined using commercial kit. L. plantarumM2 demonstrated great antimicrobial activity against all tested pathogens during 72h, namely S. aureus (96% inhibition), S. typhimurium (99% inhibition), L. monocytogenes (93% inhibition), C. utilis (48% inhibition)and E. coli(70% inhibition during 48 h). The LPS-stimulated PBMCs treated with L. plantarumM2 extracellular metabolites showed significant 66% suppression of TNF-α production (87.7 pg/mL compared to 262.5 pg/mL). Based on the results it can be assumed that donkey milk has a great potential as a source of probiotic bacteria contributing to the antimicrobial and anti-inflammatory human health benefits.

milk, antimicrobial, probiotic, immunomodulation, TNF, LPS, PBMC

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