engleski

From bio-waste to bone substitute: Biomimetic chitosan/hydroxyapatite scaffolds

The aim of the present work was comparative study of physiochemical and biological properties of obtained chitosan/hydroxyapatite scaffolds, where hydoxyapatie was obtained from three different biogenic sources. As a source of calcium ions biogenic calcium oxide, derived from hen eggshells, sea shells and cuttlefish bone was used. The synthesized powders have been characterized by elemental analysis, Fourier transform infrared spectrometry, X-ray diffraction, and Rietveld refinement studies. Highly porous chitosan/hydroxyapatite composite structures have been prepared by freeze-gelation technique, while morphology of scaffolds was imaged by scanning electron microscopy (SEM). The as-prepared powders were composed of calcium deficient hydroxyapatite and amorphous calcium phosphate. Heat treated powders were composed of hydroxyapatie, α-tricalcium phosphate and β-tricalcium phosphate. In as-prepared powders Sr2+, Mg2+ and Na+ ions were detected as result of using biogenic precursor of Ca2+ ions. SEM observations of cross section and surface area of prepared hydroxyapatite/chitosan scaffolds have shown highly porous structure with very well interconnected pores and homogeneously dispersed hydroyapatite particles. The MTT assay of scaffolds with hydroxyapatite obtained from different biogenic sources has shown no toxicity and the live dead staining has confirmed good viability, as well as proliferation of seeded cells by the culture time.

hidroksiapatit, kitozan, biogen izvor, elementi u tragovima

nije evidentirano