engleski

Evaluation of antioxidant capacity in fresh wheatgrass juice in vitro

Wheat grass juice (Triticum aestivum) is rich in active components. Fresh wheatgrass juice (WGJ) has been shown to possess a wide variety of biological activities. It has been argued that wheat grass helps general detoxification of the body due to its antioxidant potential [1]. The aim of this research was to evaluate antioxidant activity and capacity of fresh WGJ obtained from nine wheat cultivars of different origin grown in controlled conditions. The IC50 (determined by DPPH assay) in the examined samples ranged from 66.05 ± 1.03 to 106.41 ± 1.43 mg ml-1. The antioxidant capacity was evaluated on two Croatian cultivars (Felix and Ružica). Capacity was also tested on SW620 and CaCo-2 cells briefly exposed to UV radiation. Estimated relative concentrations are: SW620 1.62 mM (cv. Felix) and 1.76 mM (cv. Ružica) respectively to 1.57 mM and 1.55 mM in CaCo-2. Untreated cells show no difference in antioxidative capacity while results in exposed cells point to mild antioxidative protection. The effect on SW620 cells is 12%, and 15% to 23% on CaCo-2 cell line for cv. Ružica and cv. Felix, respectively. Obatined results point to differences between cultivars in antioxidant activity and capacity, but further analyses are necessary.

wheatgrass; ROS; cell culture; UV radiation

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