Pretražite po imenu i prezimenu autora, mentora, urednika, prevoditelja

Napredna pretraga

Pregled bibliografske jedinice broj: 1030161

Comparison of clinical and sewage isolates of Acinetobacter baumannii from two long‑term care facilities in Zagreb ; mechanisms and routes of spread


Bedenić, Branka; Siroglavić, Marko; Slade, Mia; Šijak, Dorotea; Dekić, Svjetlana; Šeruga Musić, Martina; Godan-Hauptman, Ana; Hrenović, Jasna
Comparison of clinical and sewage isolates of Acinetobacter baumannii from two long‑term care facilities in Zagreb ; mechanisms and routes of spread // Archives of microbiology, 202 (2019), 2; 301-368 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 1030161 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Comparison of clinical and sewage isolates of Acinetobacter baumannii from two long‑term care facilities in Zagreb ; mechanisms and routes of spread

Autori
Bedenić, Branka ; Siroglavić, Marko ; Slade, Mia ; Šijak, Dorotea ; Dekić, Svjetlana ; Šeruga Musić, Martina ; Godan-Hauptman, Ana ; Hrenović, Jasna

Izvornik
Archives of microbiology (0302-8933) 202 (2019), 2; 301-368

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Carbapenem ; OXA-23 ; Resistance ; Long-term care facility ; Croatia

Sažetak
In the previous studies OXA-23-like and OXA-24- like β-lactamase were reported among Acinetobacter baumannii in both hospitals and long-term care facilities (LTCF) in Croatia. The aim of this study was to analyze clinical and sewage A. baumannii isolates from two nursing homes in Zagreb, with regard to antibiotic susceptibility and resistance mechanisms, to determine the route of spread of carbapenem- resistant isolates. Nine clinical isolates were collected from February to May 2017 whereas in April 2017, ten A. baumannii isolates were collected from sewage of two nursing homes in Zagreb. Antibiotics susceptibility was determined by broth microdilution method. The presence of carbapenemase and extended spectrum β- lactamases (ESBL) encoding genes was explored by PCR. Conjugation and transformation experiments were performed as previously described. Genotyping was performed by SG determination, PFGE and MLST. Seven clinical isolates were positive for blaOXA24-like whereas two clinical and environmental carbapenem- resistant isolates, respectively, were found to possess blaOXA-23-like genes. Attempts to transfer imipenem resistance were unsuccessful indicating chromosomal location of blaOXA-23 gene. All carbapenem-resistant isolates belonged to SG- 1 (IC-2) whereas the rest of the isolates susceptible to carbapenems were allocated to SG- 2 (IC-1). PFGE analysis revealed low degree of genetic variability within both IC- I and IC- II. MLST corroborated that two environmental OXA-23 isolates belong to the ST-195. This study showed dissemination of OXA-23 producing A. baumannii from the nursing home into the urban sewage. Disinfection of nursing home sewage should be recommended in order to prevent the spread of resistance genes into the community sewage.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti



POVEZANOST RADA


Projekti:
HRZZ-IP-2014-09-5656 - Prirodno stanište klinički značajnih Acinetobacter baumannii (NATURACI) (Hrenović, Jasna, HRZZ - 2014-09) ( POIROT)

Ustanove:
Medicinski fakultet, Zagreb,
Prirodoslovno-matematički fakultet, Zagreb


Citiraj ovu publikaciju

Bedenić, Branka; Siroglavić, Marko; Slade, Mia; Šijak, Dorotea; Dekić, Svjetlana; Šeruga Musić, Martina; Godan-Hauptman, Ana; Hrenović, Jasna
Comparison of clinical and sewage isolates of Acinetobacter baumannii from two long‑term care facilities in Zagreb ; mechanisms and routes of spread // Archives of microbiology, 202 (2019), 2; 301-368 (međunarodna recenzija, članak, znanstveni)
Bedenić, B., Siroglavić, M., Slade, M., Šijak, D., Dekić, S., Šeruga Musić, M., Godan-Hauptman, A. & Hrenović, J. (2019) Comparison of clinical and sewage isolates of Acinetobacter baumannii from two long‑term care facilities in Zagreb ; mechanisms and routes of spread. Archives of microbiology, 202 (2), 301-368.
@article{article, year = {2019}, pages = {301-368}, keywords = {Carbapenem, OXA-23, Resistance, Long-term care facility, Croatia}, journal = {Archives of microbiology}, volume = {202}, number = {2}, issn = {0302-8933}, title = {Comparison of clinical and sewage isolates of Acinetobacter baumannii from two long‑term care facilities in Zagreb ; mechanisms and routes of spread}, keyword = {Carbapenem, OXA-23, Resistance, Long-term care facility, Croatia} }
@article{article, year = {2019}, pages = {301-368}, keywords = {Carbapenem, OXA-23, Resistance, Long-term care facility, Croatia}, journal = {Archives of microbiology}, volume = {202}, number = {2}, issn = {0302-8933}, title = {Comparison of clinical and sewage isolates of Acinetobacter baumannii from two long‑term care facilities in Zagreb ; mechanisms and routes of spread}, keyword = {Carbapenem, OXA-23, Resistance, Long-term care facility, Croatia} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE





Contrast
Increase Font
Decrease Font
Dyslexic Font