engleski

Expression of Inversin and Dvl-1 in developing kidneys

Introduction: Normal kidney development require complex activation of various signalling pathways. Wnt pathway is one of these pathways that is inhibited by Inversin through targeting cytoplasmic dishevelled Dvl1. The aim of this study was to explore the expression pattern of Inversin and Dvl-1 in fetal and postnatal kidney. Material & methods: The expression of Inversin and Dvl-1 was analyzed by double immunofluorescence on paraffin sections of 38- week-old fetal kidney, 1.5- and 7-years old human kidneys. The number of positive cells in proximal convoluted tubules (PCT), distal convoluted tubules (DCT) and in glomeruli were processed by ImageJ software and the data were analyzed by Kruskall Wallis and Dunn post hoc. Results: Inversin and Dvl-1 were expressed in fetal kidneys as in 1.5 and 7-year-old kidneys with different staining pattern comparing different stages and kidney structures. Inversin was highly expressed in PCT and DCT of 38-week-old fetal kidneys comparing to PCT and DCT of postnatal kidneys (P<0.001). Dvl-1 was highly expressed in PCT of fetal kidney tissue than in DCT and glomeruli of fetal kidney (P<0.01). Glomeruli of fetal kidney tissue and 1.5-year-old kidney expressed Dvl-1 higher than glomeruli of 7 years old kidney tissue (P<0.01). Conclusions: Inversin and Dvl-1 are expressed and co-localize during growth and maturation of human kidneys confirming their possible involvement in normal renal development.

Inversin ; Dvl-1 ; human embryo ; kidney ; immunofluorescence ; development

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