engleski

Storage temperature effect on total protein concentration in nose-horned viper (vipera ammodytes ammodytes) venom

Snake venoms are complex biological products containing potentially autolytic components. The aim of this pilot study was to determine the storage temperature effect on venom total protein concentration. Samples were collected by routine venom milking of three adult Nose- horned Vipers (Vipera ammodytes ammodytes). All samples were centrifuged, divided into 4 aliquots and stored for 12 hours either on 20°C, 4°C, 22°C or 37°C after which total protein concentration was measured. Our results suggest that the optimal storage temperature is 4°C and that storage on 22°C or 37°C decreases total protein concentration. Although not significantly, freezing and thawing decreases also protein concentration. Obtained results could help researchers and antivenom producers to handle and store snake venom samples appropriately. Further investigations at larger sample number, different storage times and temperatures are in progress.

Storage temperature effect, total protein concentration, nose-horned viper venom

nije evidentirano