engleski

Electrochemical Detection of Vitamin C in Real Samples

Vitamin C (ascorbic acid) is an essential, water soluble vitamin which can`t be produced by human body. It can be found in various fruits and vegetables (e.g. lemon, orange, broccoli etc.) and food supplements. The recommended daily intake is 75 mg for women and 90 mg for men. The intake of higher amounts of vitamin C can lead to health problems (gastric irritation, excessive oxidative stress, liver disease etc.). Therefore it is important to monitor daily intake of vitamin C from different sources. The aim of this study was to detect vitamin C in real samples (fruit juices and food suplements) by cyclic voltammetry. Electrochemical measurements were conducted in a three electrode voltammetric cell where glassy carbon electrode was used as a working electrode, Ag/AgCl as a reference electrode and a platinum wire as a counter electrode. The system was purged with high purity argon, Ar5 (Ar = 99.999 %) before each measurement. Vitamin C was detected in model systems where linear response was obtained in a concentration range from 0.4 mg/L to 440.0 mg/L. It was also found in real samples where its quantity varied from 2 mg/L till 64 mg/L.

vitamin C ; detection ; cyclic voltammetry ; fruit juices

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