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Optimization of ethanol/water solvent extraction of bioactive components originating from industrial hemp (Cannabis sativa L.)

Hemp (Cannabis sativa L.) containes wide range of biocompounds with differnt beneficial properties such as anti-inflammatory, antithrombotic, antiarrhythmic, hypolipidemic, and antioxidative. Response Surface Methodology (RSM) coupled with Box-Behnken design (BBD) was applied to determine the influence of the extraction temperature, solid ti liquid ratio, extraction time, rotational speed and ethanol/water solvent ratios at three levels on the solid-liquid extraction of the bioactives from hemp. Based on the obtained results, solid to liquid ratio, temperature and ethanol content had statistically significant effects on the total polyphenolic content (TPC), while extraction time and rotational speed had non influence on the TPC extraction. Regarding antioxidant activity determined by DPPH method, only solid to liquid ratio had statistically significant effect, with no influence of other process variables. Solid to liquid ratio, ethanol content, temperature and rotational speed significantly influenced antioxidant activity determined by FRAP method. According to BBD, the optimum extraction conditions were as follows: extraction temperature 45 °C, solid to liquid ratio 30 mL g-1, extraction time 25 min, rotational speed 500 rpm, etanol content 25 %. RSM coupled with a BBD was shown to be effective for modeling and optimization the solid-liquid extraction of hemp.

hemp (Cannabis sativa L.), solid-liquid extraction, optimization, bioactives

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