engleski

Activation status, Toll like receptor 4 and CD91 expression of synovial tissue macrophages in patients with mature osteoarthritis

The quantity of activated macrophages in synovial tissue is associated with radiographic severity of knee osteoarthritis (OA) and joint symptoms. We aimed to investigate activation status, Toll like receptor (TLR)4 and CD91 expression of synovial tissue macrophages in patients with mature osteoarthritis. We sampled synovial tissue during the knee alloarthroplasty. Interleukin 15, interferon- gamma, TLR4, CD91, lineage and activation markers were labeled by immunohistology and/or immunofluorescence in paraffin embedded serial tissue sections. Three investigators counted CD68+ cells in synovial sections independently under the microscope. The average number of synovial CD68+ cells per microscope field 400 was 35  21 with no statistically significant difference in the numbers among observers. CD68+ macrophages surround the vessels and spread toward the surface of synovial shoots. Their distribution was similar to the distribution of CD14, CD91 and TLR 4. The cells expressing CD68, HLA-DR and interleukin-15 appoint in synovial lymphocyte accumulation, nearby CD3+ and CD56+ cells. Synovial tissue of OA patients nests interferon-gamma+ cells in certain parts. Conclusion: Peripheral blood derived CD68+ synovial macrophages, equipped with TLR4/CD14 and CD91 pattern recognition receptors possibly interact with synovial fluid antigens, which might support their M1 maturation characterized by intense interleukin-15 and HLA-DR expression, as well as their interaction with synovial lymphocytes, particularly in the parts rich with interferon- gamma

osteoarthritis ; synovial tissue ; macrophages ; toll-like receptor 4 ; CD91

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