engleski

Diversity of Vibrio spp. in farmed and wild fish of Mali Ston Bay, Pelješac (Croatia)

The genus Vibrio consists of more than 100 species, a number of which could be pathogenic for humans. Besides in molluscs, Vibrio spp. can also be found in fish which can represent a risk to human health. In this work we present the diversity of Vibrio spp. in farmed and wild fish from the Adriatic Sea in the Malostonski Bay. Total of 51 European seabass (Dicentrarchus labrax) from the fish farm and 37 wild fish caught around cages were sampled for microbiological assessment of Vibrio spp. Gills and skin swabs (below dorsal fin) were taken using sterile swab sticks, swabbing an area of 1 cm2. After swab sampling, wild fish were returned alive to the sea. Samples were inoculated on the Marine agar and TCBS agar. Plates were incubated on 22 °C and 35 °C and the number was estimated as CFU for 1 cm2 of gills and skin. Nine different Vibrio species were found in farmed European seabass samples. The most frequent were V. alginolyticus, V. anguillarum and V. parathaemolyticus. Other six species were present in less than five samples (V. chagasii, V. fortis, V. harveyi, V. mytili, V. scophthalmi and V. tasmaniensis). In wild seabass, six different Vibrio species were found but only V. harveyi was found in more than one sample and it was present in the highest number in other wild fish species, folowed by V. ichthyoenteri. Other present Vibrio spp. in wild fish were V. alginolyticus, V. chagasii, V. furnissii, V. gigantis, V. neptunius, V. orientalis, V. parahaemolyticus, V. pomeroyi, V. rotiferianus and V. tasmaniensis. Highest Vibrio spp. diversity was present in farmed fish, but we also found high diversity in different wild fish species, although fewer in number. Beside proven pathogen V. parathaemolyticus, we found other Vibrio species that may cause harm to human health. These findings indicate necessity to follow good hygiene practice in cases fish are intended for human consumption and to monitor Vibrio spp. regularly.

Diversity, Vibrio spp., farmed and wild fish

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