hrvatski

Salivary squamous cell carcinoma antigen 1 and 2 in patients with leukoplakia: A pilot study

Aim: Leukoplakia is the most common potentially malignant oral disorder (PMOD) with a worldwide prevalence of 2% and a malignant transformation rate of 0.3%. Squamous cell carcinoma antigens (SCCA) are part of the serpins family. They are involved in inflammation, apoptosis, cell- migration and are expressed in the squamous cell epithelium. SCCA2 protein is 92% homologous to SCCA1, however these antigens are not expressed concurrently and play different roles in the human body. Their role in the healthy and malignant tissues is not clearly understood. Materials and methods: Unstimulated whole saliva (UWS) and stimulated whole saliva (SWS) were sampled from 5 patients with leukoplakia (5 male) and 12 age and sex matched control subjects. Leukoplakia was diagnosed with a C2certainty factor. Sandwich human SCCA1 and SCCA2 ELISA Kits, My BioSource, San Diego, USA, were used. Respondents’ alcohol consumption, papilla bleeding index (PBI), smoking, drug consumption and medical condition was registered. This study has been funded by the Croatian Science Foundation (IP-09-2014- 9376). Results: Salivary SCCA1 was significantly higher in patients with leukoplakia in UWS (U=4, p=0.045). No statistically significant difference in SCCA2 levels in UWS and SWS between the two groups was found. Due to the small sample size, smoking, PBI, medical conditions, drug and alcohol consumption had no statistical significance. Conclusion: Salivary SCCA1 could serve as a satisfactory biomarker for leukoplakia. However, a greater sample is needed to establish the diagnostic value of the investigated molecules. To our knowledge, SCCA1 and SCCA2 have not yet been measured in patients with leucoplakia.

salivary squamous cell carcinoma ; antigen 1 and 2 ; leukoplakia

nije evidentirano

engleski

Salivary squamous cell carcinoma antigen 1 and 2 in patients with leukoplakia: A pilot study

Aim: Leukoplakia is the most common potentially malignant oral disorder (PMOD) with a worldwide prevalence of 2% and a malignant transformation rate of 0.3%. Squamous cell carcinoma antigens (SCCA) are part of the serpins family. They are involved in inflammation, apoptosis, cell- migration and are expressed in the squamous cell epithelium. SCCA2 protein is 92% homologous to SCCA1, however these antigens are not expressed concurrently and play different roles in the human body. Their role in the healthy and malignant tissues is not clearly understood. Materials and methods: Unstimulated whole saliva (UWS) and stimulated whole saliva (SWS) were sampled from 5 patients with leukoplakia (5 male) and 12 age and sex matched control subjects. Leukoplakia was diagnosed with a C2certainty factor. Sandwich human SCCA1 and SCCA2 ELISA Kits, My BioSource, San Diego, USA, were used. Respondents’ alcohol consumption, papilla bleeding index (PBI), smoking, drug consumption and medical condition was registered. This study has been funded by the Croatian Science Foundation (IP-09-2014- 9376). Results: Salivary SCCA1 was significantly higher in patients with leukoplakia in UWS (U=4, p=0.045). No statistically significant difference in SCCA2 levels in UWS and SWS between the two groups was found. Due to the small sample size, smoking, PBI, medical conditions, drug and alcohol consumption had no statistical significance. Conclusion: Salivary SCCA1 could serve as a satisfactory biomarker for leukoplakia. However, a greater sample is needed to establish the diagnostic value of the investigated molecules. To our knowledge, SCCA1 and SCCA2 have not yet been measured in patients with leucoplakia.

salivary squamous cell carcinoma ; antigen 1 and 2 ; leukoplakia

nije evidentirano