Three-Year Experience in NAT Screening of Blood Donors for Transfusion Transmitted Viruses in Croatia (CROSBI ID 269713)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Safić Stanić, Hana ; Babić, Ivana ; Maslović, Margareta ; Dogić, Vesna ; Bingulac-Popović, Jasna ; Miletić, Manuela ; Juraković-Lončar, Nina ; Vuk, Tomislav ; Strauss-Patko, Maja ; Jukić, Irena
engleski
Three-Year Experience in NAT Screening of Blood Donors for Transfusion Transmitted Viruses in Croatia
Croatia implemented individual donation (ID)- NAT testing of blood donors in 2013 for three viruses HBV, HCV, and HIV-1 as a mandatory test for all blood donors. This study assessed the impact of NAT screening 3 years after its implementation. Methods: A total of 545, 463 donations were collected and screened for HBV, HCV, and HIV-1 using the Procleix Ultrio Plus Assay. All initially reactive (IR) NAT samples were retested in triplicate and, if repeatedly reactive (RR), NAT discriminatory assay (dNAT) was performed. ID-NAT positive donations were confirmed by RT-PCR on the COBAS AmpliPrep/TaqMan platform. Results: Out of 545, 463 samples tested, 108 (0.02%) were RR in NAT. There were 82 (75, 9%) HBV reactive, 16 (14.8%) HCV reactive, and 10 (9.3%) HIV-1 reactive samples. 51 (47.2%) samples were ID-NAT positive only. Out of these 51 NAT yield cases, 1 window period HIV-1 and 50 occult HBV infections (OBI) were determined. There were only two potential HBV DNA transmissions from OBI donors. Conclusion: The implementation of NAT screening for three viruses has improved blood safety in Croatia. During the 3-year period, 1 window period HIV-1 and a number of occult HBV donations were identified.
Blood donor’s screening ; NAT testing ; Transfusion-transmissible infections
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Podaci o izdanju
44
2017.
415-420
objavljeno
1660-3796
1660-3818
10.1159/000457965
Povezanost rada
Kliničke medicinske znanosti