engleski

Crystallization and X-ray crystallographic studies on insulin derivatives

Insulin is a hormone protein which is produced in the -cells in the Islets of Langerhans, located in the pancreas. Owing to its crucial metabolic role and its pharmaceutical importance many structural studies on chemically and genetically modified insulins have been done. In this talk, crystallization and single crystal X-ray crystallographic studies of modified insulin hexamers will be presented. Replacement of zinc ions with selected essential elements: copper, nickel, manganese and cobalt has been done and the prepared insulin derivatives were structurally characterized. All of the investigated insulin derivatives belong to the T6 form (Fig. 1a). [1]−[3] Crystallization of insulin in the presence of chloride anions resulted in new crystal forms of the cobalt and nickel T6 insulin derivatives. [4] Derivatives of the insulin hexamers that were crystallized in the presence of bromide (Fig. 1b), T3R3f form, and iodide ions, T6 form (Fig. 1c) have also been prepared and structurally characterized.[5] Coordination of zinc ions and conformation of the insulin molecule in all investigated insulin hexamers will be discussed.

crystallization ; X-ray crystallography

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