engleski

Silver-modified zeolite in bioremediation of soil contaminated with Acinetobacter baumannii

Bacterium Acinetobacter baumannii is an opportunistic pathogen of great clinical significance. Clinically significant A. baumannii isolates have been recorded in wastewaters, natural aquatic habitats and soils contaminated by human waste. The aim of this study was to investigate the potential application of unmodified (NZ) and Ag-modified (AgNZ) natural zeolitized tuff to remove A. baumannii from natural soil. Pure culture of pandrug-resistant A. baumannii was suspended in spring water and inoculated into 100 g of fresh alkaline red palaeosol, which was supplemented with 1 wt.% of NZ or AgNZ. The abundance of A. baumannii was monitored on CHROMagar Acinetobacter at 36 °C/24h, while total heterotrophic bacteria were monitored on Nutrient agar 22 °C/72h. A. baumannii successfully survived 50 days in soil with the addition of NZ. The AgNZ showed a strong bactericidal activity, after only 1h exposure (removal of 6.8±0.1 log CFU/g). The abundance of native heterotrophic bacteria was reduced by AgNZ for 2.6±0.1 log CFU/g, but a large portion of the population remained viable (4.1±0.2 log CFU/g). AgNZ is a promising material for the bioremediation of soil contaminated with hospital pathogen A. baumannii. The application of AgNZ does not influence the population of native heterotrophic bacteria in soil, which makes it acceptable for use in real environment.

Acinetobacter baumannii, bacteria, soil, natural zeolite, Ag-modified zeolite

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