engleski

Protective effect of Rosmarinus officinalis L. and Taraxacum officinale L. extracts on genetic material of human squamous carcinoma cell line CAL27

Rosemary and dandelion plants are commonly used in Mediterranean diet but because of their anti-inflammatory and protective properties they are increasingly used in prevention and reduction of various diseases. The aim of this study was to test the effect of different concentrations of rosemary and dandelion leaves extracts (0.05x-5x, where 1x is the concentration in a single cup of therapeutic beverage) on the viability and primary (alkaline comet assay) and permanent (micronucleus cytome assay) genomic damage to human squamous carcinoma cell line CAL27 compared to the control (C). Also, protective effect of both extract against oxidative stress was determined. Comet assay showed that only 1-hour treatment with 5x concentrated dandelion extracts caused a statistically significant change in tail intensity (%DNA in tail ± SE = 10.39 ± 13.38 ; C = 2.39 ± 4.07). On the other hand, rosemary extract was more influential on genetic material at lower concentrations (1x, 2.5x and 5x) than dandelion (%DNA in tail ± SE = 6.75 ± 9.08 ; 7.24 ± 9.20 ; 16.73 ± 11.70 ; 2.39 ± 4.07 (C)). Also, micronucleus cytome assay showed that concentrations 0.05x and 0.1x of both extracts with 4-hours exposure caused a statistically significant increase in micronucleus, while higher concentrations induced apoptosis and necrosis. When exposed to lower concentration of hydrogen peroxide (0.25 μM), rosemary extract reduced the fragmentation and percentage of DNA damage at all working concentrations (min%DNA in tail ± SE = 16.14 ± 13.09 ; max%DNA in tail ± SE = 24, 32 ± 16.86 ; PC = 39.88 ± 22.19) and its potential role in decreasing oxidative damage was confirmed. At higher hydrogen peroxide dose (0.50 μM), rosemary extract showed a protective effect by decreasing the number of cells in apoptosis. Dandelion extract did not exhibit any protective effect. Both test extracts are presented as powerful agents that can be found in various therapeutic treatments but should be careful in dosage as the concentration in a cup of tea has already shown an apoptotic effect.

protective effect ; rosemary ; dandelion ; comet assay ; micronucleus cytome assay

nije evidentirano