engleski

Determination of therapeutic imidazole dipeptides in meat by microchip electrophoresis

Miniaturization of the capillary electrophoresis (CE) devices, enables fast and low-cost analysis with low sample consumption. In this study we introduced a home–made compact and rugged low–cost microchip electrophoresis (MCE) instrument with high throughput. MCE instrument with capacitively coupled contactless conductivity detector (C4D) was applied for the separation of carnosine and anserine. Carnosine and its metabolic derivative anserine are bioactive imidazole dipeptides found in the skeletal tissue of mammals and birds. Imidazole dipeptides perform multiple physiological functions: pH buffering, membrane–protecting activity, anti-oxidation, anti-glycation and anti-inflammation. Carnosine has preventive, protective and therapeutic effects in treatment of autism, diabetes, ischemia, hepatic diseases and neurodegenerative diseases and also have an anti-proliferative effect on cancer cells in vivo. The C4D detector responded linearly to both imidazole dipeptides in the range 0–20 mg L−1. Standard addition method was used to determine the accuracy and precision of the method. The method was also applied to real meat samples originated from different animal species. The results suggested that the MCE-C4D method is feasible for rapid analysis of animal-derived samples with satisfying precision and accuracy. Imidazole dipeptides are usually determined by HPLC using various detection principles. The proposed MCE-C4D method represents a cheap, fast, and simple alternative to the existing HPLC methods. C4D detection is a universal electrochemical detection method which does not demand bulky optical detectors nor labeling of the sample components prior to detection. This method can be used as a method for detection, species identification, and quantification of animal imidazole dipeptides in raw meat. Low- cost and portable MCE instrument with a short analysis time makes this method a promissing tool for food testing and in food adulteration prevention process, especially in developing countries. This method can also be applied to test the presence of animal proteins in vegetable feeds.

C4D detector, imidazole dipeptides, microchip electrophoresis

nije evidentirano