engleski

Separation of bioactive compounds from tobacco waste using the sequence of supercritical CO2 extraction and subcritical water extraction

The food and plant industry generate large amounts of wastes or by-products annually around the world from a variety of sources. Improper management and illegal dumping of waste, particularly hazardous and toxic waste, possess increasing threats to the environment and human health. Tobacco waste is a by-products produced in large quantities during processing of tobacco and in most cases, represents an inefficient use of valuable compounds. Tobacco waste (type: scrap) in this study was obtained from “Fabrika Duhana Sarajevo” from Bosnia and Herzegovina. A series of operational parameters of supercritical CO2 (SC- CO2) extraction of tobacco waste (pressure: 100– 300 bar, temperature: 40–80 °C), were investigated in a laboratory scale apparatus in order to get extracts rich in nicotine. The CO2 mass flow rate was kept constant during the process. From reused tobacco waste (after SC-CO2 extraction), subcritical water extraction (SWE) under selected conditions (solvent/solid ratio: 28 mL/g, temperature: 150 ˚C, time: 23 min) was sequentially performed to obtain the extracts rich in phenolic compounds. The content of bioactive compounds was determined using High Performance Liquid Chromatography (HPLC) and by gas chromatography (GC-MS) Obtained extracts of tobacco waste after SC-CO2 extraction were characterized by high levels of nicotine, and after SWE extracts were characterized by a high content of phenolic compounds, considerable amounts of nicotinic acid and nicotinamide and reduced content of nicotine. Also, presence of 5- HMF, furfural and 5-MF was detected in SWE extracts.

tobacco waste ; supercritical CO2 extraction ; subcritical water extraction

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