engleski

Cadmium influx across isolated Carcinus gill epithelium-Interaction of lanthanum and calcium with cadmium influxes

The effects of Ca and La on Cd transport across apical and basolateral gill surfaces were examined in isolated perfused Carcinus gills.In the gills exposed to diluted sea water enriched with calcium by addition of CaCl2 to 11.9 and 15.9 mmol/l the Cd influxes decreased by 55% and 85%, respectively, compared to those observed in diluted sea water (6.9 mmol Ca/l). When Ca was added to the perfusate to increase its concentration at the basolaterally oriented gill surfaces, Ca influx inhibition was less pronounced than when Ca was added apically. The effect of Ca on Cd-109 influxes appears, therefore, to be exerted at the apical gill surface. Additionally, the effects of various concentrations on the non- specific Ca channekl blocker La (added to diluted sea water) on Cd-109 influxes in isolated perfused Carcinus gills were studied. Cd-109 are shown to be lanthanum concentration-dependent. The half- maximum inhibition of cadmium influxes by La occured at 1.4 x 10 mol/l. Cadmium transport is discussed in termds of non-specific influx utilizing Ca channels.

perfused gills ; Cd influx ; Ca and La interactions ; vrab ; carcinus

nije evidentirano