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Pregled bibliografske jedinice broj: 1021100

The expression level changes of lineage-specific cell surface molecules on innate immune cells in response to orthohantavirus infection


Svoboda, Petra; Cvetko Krajinović, Lidija; Polančec, Denis; Kurolt, Ivan Christian; Mačak Šafranko, Željka; Markotić, Alemka
The expression level changes of lineage-specific cell surface molecules on innate immune cells in response to orthohantavirus infection // Annual Meeting of Croatian Immunological Society - Book of abstracts
Zadar, Hrvatska, 2018. str. 59-59 (poster, domaća recenzija, sažetak, znanstveni)


Naslov
The expression level changes of lineage-specific cell surface molecules on innate immune cells in response to orthohantavirus infection

Autori
Svoboda, Petra ; Cvetko Krajinović, Lidija ; Polančec, Denis ; Kurolt, Ivan Christian ; Mačak Šafranko, Željka ; Markotić, Alemka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Annual Meeting of Croatian Immunological Society - Book of abstracts / - , 2018, 59-59

Skup
Annual Meeting of Croatian Immunological Society

Mjesto i datum
Zadar, Hrvatska, 19.-20.10.2018

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
Primary human monocytes, macrophages, dendritic cells, immunophenotyping

Sažetak
Introduction: Puumala and Tula orthohantavirus are enveloped RNA viruses, belonging to genus Orthohantavirus (HTV), family Hantaviridae, order Bunyavirales. Puumala orthohantavirus (PUUV) is pathogenic causing mild to moderate forms of hemorrhagic fever with renal syndrome in Euroasia while Tula orthohantavirus (TULV) is considered apathogenic due to limited evidence of pathogenesis in humans. Monocytes, macrophages and dendritic cells (DC), as innate immune system cells, are target cells for HTV potentially contributing to dissemination of the virus in the body and development of the disease. There is still a lack of knowledge about the mechanisms of immune response to HTV. The aim of this in vitro study was to analyze the expression dymanics of the selected cell surface molecules on monocytes, macrophages and DC infected with PUUV or TULV in order to investigate whether orthohantavirus infection triggers differentiation and subsequent cell polarization. Materials and Methods: Primary human monocytes were infected in vitro with PUUV or TULV and cultured for seven days post infection. Immunophenotyping was done in three time points using in-house created polychromatic screening panel with antibodies specific for surface molecules on monocytes, macrophages and DC. After staining and fixation, samples acquisition was performed on a Navios flow cytometer (Beckman Coulter, USA). Data files were analysed using FlowLogic software (Inivai Technologies, Australia). Results: The expression levels of lineage-specific (monocyte, macrophage and DC) cell surface molecules between orthohantavirus infected cells and mock infection were different, as well as between pathogenic and apathogenic infections. Conclusion: Orthohantaviruses induce differentiation of primary human monocytes as seen at the level of cell surface markers expression. Differences are observed between pathogenic and apathogenic orthohantaviruses infections.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti



POVEZANOST RADA


Ustanove
Klinika za infektivne bolesti "Dr Fran Mihaljević",
Dječja bolnica Srebrnjak,
Hrvatsko katoličko sveučilište, Zagreb

Citiraj ovu publikaciju

Svoboda, Petra; Cvetko Krajinović, Lidija; Polančec, Denis; Kurolt, Ivan Christian; Mačak Šafranko, Željka; Markotić, Alemka
The expression level changes of lineage-specific cell surface molecules on innate immune cells in response to orthohantavirus infection // Annual Meeting of Croatian Immunological Society - Book of abstracts
Zadar, Hrvatska, 2018. str. 59-59 (poster, domaća recenzija, sažetak, znanstveni)
Svoboda, P., Cvetko Krajinović, L., Polančec, D., Kurolt, I., Mačak Šafranko, Ž. & Markotić, A. (2018) The expression level changes of lineage-specific cell surface molecules on innate immune cells in response to orthohantavirus infection. U: Annual Meeting of Croatian Immunological Society - Book of abstracts.
@article{article, year = {2018}, pages = {59-59}, keywords = {primary human monocytes, macrophages, dendritic cells, immunophenotyping}, title = {The expression level changes of lineage-specific cell surface molecules on innate immune cells in response to orthohantavirus infection}, keyword = {primary human monocytes, macrophages, dendritic cells, immunophenotyping}, publisherplace = {Zadar, Hrvatska} }