engleski

Growth inducing properties of the marine organisms extracts on selected fungal species

Even though marine organisms are known for their bioactive compounds having beneficial effect on human body, the compounds with opposite activity are also present in such organisms. Therefore, this study aimed to investigate the growth inducing effect of the marine organisms extracts on the tested fungi. Five different marine organisms including Aplidium conicum, Codium bursa, Cystoseira sp. and Eunicella cavolinii, all collected in April 2018 from the Adriatic Sea, were used for the extraction process. The extracts of marine organisms were obtained with ultrasound-assisted extraction (UAE) which was performed in the ultrasound bath with two different solvents, water and dimethylsulfoxide (DMSO). Polar compounds were extracted with water, while non-polar compounds were obtained with DMSO. The prepared extracts were used for the testing of growth inducing properties on the most problematic mycotoxigenic fungal species such as Penicillium expansum, Fusarium verticilloides, Aspergillues flavus, Alternaria alternate, Aspergillus ochraceus and Fusarium gramniearum. The standardized microdilution method used for the testing was in accordance with the NCCLS guidelines, document M38-A. According to the results, there is a significant difference in the growth inducing properties on tested fungal species depending on the used extract and their concentration. Some of the extracts showed growth inducing properties at low concentrations (5 and 50 μg/mL), while in some cases the high amount of DMSO or H2O extract (5000 μg/mL) promoted growth of the fungi.

marine organisms ; growth inducing potential ; fungal species

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