engleski

Kidney injury triggered by an intravascular hemolysis in fish – a multilevel approach

Intravascular hemolysis can result in hemoglobinuria with acute kidney injury in fish after antiparasitic treatment. The objective of this work was to study the cumulative effect of organophosphate azamethiphos on clinical chemistry, tissue histopathology and proteome expression in rainbow trout grown in a commercial farm, to determine its effect on health status after repeated treatments. In this study we systematically explored fish as an animal model and we compared the results from a study using multilevel approach in order to identify hemoglobinuria­triggered damage pathways. The tissue damage after the treatments showed increased deposits of hemosiderin in kidney and spleen. Tubule cells of hemolytic animals demonstrated enhanced iron deposits. Conformation proteome analysis revealed decreased haptoglobin in fish serum. Results from this study indicate the possible impact of azamethiphos treatment on trout health through intravascular hemolysis caused by the treatment, and consequently from pathophysiologic process of hemoglobin metabolism and its products causing chronic kidney injury from iron deposits. Specifically, the important aspect of this study is the fact that it evaluates the importance of toxic potential from excessive red blood cells breakdown. Impact of active iron accumulated in different organs from physiological processes is the first report of that kind in fish that can seriously impair normal function. In addition, this study enabled validation of serum clinical chemistry assessment based on variety of different endpoints. This experiment confirmed that free heme is a likely to trigger of tubule barrier deregulation and oxidative cell damage reinforcing the hypothesis that uncontrolled free heme could trigger significantprotein value response as an important pathway of renal injury during intravascular hemolysis.

fish ; kidney ; azamethiphos ; histology ; iron ; proteome

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