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Effects of silver nanoparticles and silver nitrate on photosynthesis and photosynthesis-related proteins in tobacco (Nicotiana tabacum) – a comparative study (CROSBI ID 680881)

Prilog sa skupa u časopisu | sažetak izlaganja sa skupa | međunarodna recenzija

Tkalec, Mirta ; Peharec Štefanić, Petra ; Biba, Renata ; Cvjetko, Petra ; Šikić, Sandra ; Balen, Biljana Effects of silver nanoparticles and silver nitrate on photosynthesis and photosynthesis-related proteins in tobacco (Nicotiana tabacum) – a comparative study // Toxicology letters / Dekant, Wolfgang (ur.). 2019. str. S200-S200

Podaci o odgovornosti

Tkalec, Mirta ; Peharec Štefanić, Petra ; Biba, Renata ; Cvjetko, Petra ; Šikić, Sandra ; Balen, Biljana

engleski

Effects of silver nanoparticles and silver nitrate on photosynthesis and photosynthesis-related proteins in tobacco (Nicotiana tabacum) – a comparative study

The small size of nanoparticles (NPs), with dimensions between 1 and 100 nm, results in unique chemical and physical characteristics, which is why they are being implemented in various consumer products. Therefore, one of the important concerns is the potential detrimental impact of NPs on environment. Among different types of available nanomaterials, the most frequently applied are silver nanoparticles (AgNPs) due to antimicrobial properties of silver. As plants are the vital part of ecosystem and the first component of the food chain, the investigation of NPs phytotoxic effects is of particular interest. In this study, we examined the effects of citrate-coated AgNPs and its bulk form (AgNO3) on photosynthesis and leaf proteome of tobacco plants exposed to 100 µM AgNPs and AgNO3 for 7 days. Silver accumulation in leaf tissue was determined by ICP-MS. Changes in photosynthesis were evaluated by measuring the chlorophyll fluorescence parameters, while content of photosynthetic pigments was analysed by HPLC. Two-dimensional gel electrophoresis (2-DE) and MALDI mass spectrometry were employed to reveal the changes in protein expression. Both types of treatments resulted with the similarly increased Ag uptake. Significantly decreased photochemical quenching and increased concentration of violaxantin was recorded after exposure to AgNPs. On the contrary, treatments with AgNO3 did not significantly influence fluorescence parameters, although they induced a negative effect on majority of photosynthetic pigments. Identified proteins with differential expression were found to be mostly photosynthesis-related and down-regulated, although almost half of the proteins exhibited different expression level between AgNPs and AgNO3 exposure. Obtained results indicate that the AgNPs effects observed in tobacco leaves are not simply due to the release of silver ions and can be correlated with distinct impact of silver nanoparticle form.

chlorophyll fluorescence, nanosilver, photosynthetic pigments, proteomics

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Podaci o prilogu

S200-S200.

2019.

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objavljeno

Podaci o matičnoj publikaciji

Toxicology letters

Dekant, Wolfgang

Helsinki: Elsevier

0378-4274

Podaci o skupu

55th Congress of the European Societies of Toxicology (EUROTOX 2019)

poster

08.09.2019-11.09.2019

Helsinki, Finska

Povezanost rada

Biologija, Kemija

Indeksiranost