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Pregled bibliografske jedinice broj: 1020514

Lipophilicity and bio‐mimetic properties determination of phytoestrogens using ultra‐ high‐performance liquid chromatography


Lasić, Kornelija; Bokulić, Ana; Milić, Astrid; Nigović, Biljana; Mornar, Ana
Lipophilicity and bio‐mimetic properties determination of phytoestrogens using ultra‐ high‐performance liquid chromatography // Biomedical chromatography, 33 (2019), e4551, 13 doi:10.1002/bmc.4551 (međunarodna recenzija, članak, znanstveni)


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Naslov
Lipophilicity and bio‐mimetic properties determination of phytoestrogens using ultra‐ high‐performance liquid chromatography

Autori
Lasić, Kornelija ; Bokulić, Ana ; Milić, Astrid ; Nigović, Biljana ; Mornar, Ana

Izvornik
Biomedical chromatography (0269-3879) 33 (2019); E4551, 13

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Caco‐2 cells ; Immobilized artificial membrane chromatography ; Lipophilicity ; Phytoestrogens ; Protein binding

Sažetak
This paper presents lipophilicity and bio‐ mimetic property determination of 15 phytoestrogens, namely biochanin A, daidzein, formononetin, genistein, genistein‐ 4, 7‐dimethylether, prunetin, 3, 4, 7‐ trihydroxyisoflavon, 4, 6, 7‐trihydroxyisoflavon, 4, 6, 7‐trimethoxyisoflavon, daidzin, genistin, ononin, sissotrin, coumestrol and coumestrol dimethylether. High‐performance liquid chromatography with fast gradient elution and Caco‐2 cell line were used to determine the physicochemical properties of selected phytoestrogens. Lipophilicity was determined on octadecyl‐sylane stationary phase using pH2.0 and pH 7.4 buffers. Immobilized artificial membrane chromatography was used for prediction of interaction with biological membranes. Protein binding was measured on human serum albumin and α‐1‐acid‐glycoprotein (AGP) stationary phases. Caco‐2 assay was used as a gold standard for assessing in vitro permeability. The obtained results differentiate phytoestrogens according to their structure where aglycones show significantly higher lipophilicity, immobilized artificial membrane partitioning, AGP binding and Caco‐2 permeability compared with glucosides. However, human serum albumin binding was very high for all investigated compounds. Furthermore, a good correlation between experimentally obtained chromatographic parameters and in silico prediction was obtained for lipophilicity and human serum albumin binding, while the somewhat greater difference was obtained for AGP binding and Caco‐2 permeability.

Izvorni jezik
Engleski

Znanstvena područja
Farmacija



POVEZANOST RADA


Ustanove
Farmaceutsko-biokemijski fakultet, Zagreb,
Pliva-Istraživački institut,
Fidelta d.o.o.

Profili:

Avatar Url Ana Bokulić (autor)

Avatar Url Biljana Nigović (autor)

Avatar Url Ana Mornar Turk (autor)

Avatar Url Astrid Milić (autor)

Citiraj ovu publikaciju

Lasić, Kornelija; Bokulić, Ana; Milić, Astrid; Nigović, Biljana; Mornar, Ana
Lipophilicity and bio‐mimetic properties determination of phytoestrogens using ultra‐ high‐performance liquid chromatography // Biomedical chromatography, 33 (2019), e4551, 13 doi:10.1002/bmc.4551 (međunarodna recenzija, članak, znanstveni)
Lasić, K., Bokulić, A., Milić, A., Nigović, B. & Mornar, A. (2019) Lipophilicity and bio‐mimetic properties determination of phytoestrogens using ultra‐ high‐performance liquid chromatography. Biomedical chromatography, 33, e4551, 13 doi:10.1002/bmc.4551.
@article{article, year = {2019}, pages = {13}, DOI = {10.1002/bmc.4551}, chapter = {e4551}, keywords = {Caco‐2 cells, Immobilized artificial membrane chromatography, Lipophilicity, Phytoestrogens, Protein binding}, journal = {Biomedical chromatography}, doi = {10.1002/bmc.4551}, volume = {33}, issn = {0269-3879}, title = {Lipophilicity and bio‐mimetic properties determination of phytoestrogens using ultra‐ high‐performance liquid chromatography}, keyword = {Caco‐2 cells, Immobilized artificial membrane chromatography, Lipophilicity, Phytoestrogens, Protein binding}, chapternumber = {e4551} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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