engleski

RecBCD- RecFOR-independent pathway of homologous recombination in Escherichia coli

A novel RecBCD- RecFOR-independent (RecBFI) recombination pathway is identified in E. coli. It operates in a specific genetic background, i.e. in sbcB15 sbcCD mutants. The RecBFI pathway strongly depends on recA, recJ and recQ gene functions, and moderately depends on recG and lexA functions. The key role in this pathway is played by the mutant SbcB15 protein (a defective ExoI that retained DNA-binding activity). SbcB15 facilitates loading of the RecA protein in the absence of functional RecBCD and RecFOR proteins.

Recombination pathways ; RecBCD ; RecFOR ; sbcB15 mutation ; transduction ; DNA repair

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