engleski

BIOLOGICAL ACTIVITIES OF ESSENTIAL OIL AND HYDROLATE OF Hypericum perforatum L. ssp. veronense (Schrank) H. Lindb.

Chemical composition, antiphytoviral activity and cytotoxicity of Hypericum perforatum ssp. veronense (Schrank) H. Lindb. (Clusiaceae) essential oil and hydrolate derived from the aerial parts of the plant is presented. Forty- six compounds, representing 82.5% of the oil, were identified. GC-MS analyses of the oil revealed presence of α-pinene (16.5%), n-nonane (13.5%), (E)-caryophyllene (9.5%) and caryophyllene oxide (7.6%) as the main oil components. HPLC analysis identified 3, 4- dihydroxybenzoic acid (22.66 μg/mL) as the main component of the hydrolate. Both essential oil and hydrolate of H. p. ssp. veronense increased the plant resistance to viral infection with tobacco mosaic virus. Local host plants treated with essential oil prior to virus inoculation showed delay in the development and almost complete reduction of lesions in the early stage of infection. Pretreatment by hydrolate reduced the number of lesions on the leaves of local host plants as well as the virus concentration in systemically infected plants. In order to assess the antiproliferative potential of H. p. ssp. veronense, cytotoxicity for hydrolate was determined on healthy (RPE1) and tumor (HeLa) cell lines. The obtained results reveal similar susceptibility toward both cell lines, ensuring further investigation of both, essential oil and hydrolate, on more cell lines to enable further elucidation of H. p. ssp. veronense biological activity. This study opens up a new area of research concerning plant protection against viruses by demonstrating not only antiphytoviral activity of essential oils, but also aromatic plant hydrolates, with potential application in development of new naturally derived antiphytoviral preparations.

antiphytoviral activity, cytotoxicity, tobacco mosaic virus, 3, 4-dihydroxybenzoic acid

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