engleski

Antimicrobial resistance of Yersinia enterocolitica O:3 isolated from tonsils and lymph nodes of slaughtered pigs in Croatia

Antimicrobial resistance of foodborne pathogens presents one of the main public health concerns. In this survey, phenotypic antimicrobial resistance was evaluated in Yersinia enterocolitica isolated from tonsils and lymph nodes of slaughtered pigs in Croatian slaughterhouses. Presumptive colonies (n=49) were selected from CIN and CHROMagarTM Y.enterocolitica and subjected to MALDI-TOF MS identification and serotyping. Antimicrobial susceptibility was tested by disk diffusion method toward levofloxacin, ciprofloxacin, ampicillin, cephalothin, cefotaxime, tetracycline, nalidixic acid, ceftazidime, trimethoprim/sulfamethoxazole, chloramphenicol and streptomycin. Resistance of strains was assessed following EUCAST/CLSI guidelines. All strains were confirmed by MALDI-TOF MS as Yersinia enterocolitica and belonged to O:3 serotype. The majority of strains was resistant toward ampicillin (91.6 %) and cephalothin (85.4 %), followed by chloramphenicol (31.2 %), nalidixic acid (31.2 %), streptomycin (27.0 %), tetracycline (8.3%) and trimethoprim/sulfamethoxazole (2.0 %). Only one strain was susceptible to all antimicrobial agents tested. Y. enterocolitica strains of pigs from medium-scale farms were mostly resistant to ampicillin and cephalothin, while strains collected from large farms were additionally resistant to chloramphenicol, nalidixic acid and streptomycin. Multiresistance (resistance to three or more agents) was found in 17 strains (35.4 %). Higher prevalence of multiresistant Y. enterocolitica was evident in pigs originated from large farms.

Yersinia enterocolitica, AMR, pigs, tonsils, lymph nodes, VPH

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