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Pregled bibliografske jedinice broj: 1015868

Azithromycin-liposomes as a novel approach for localized therapy of cervicovaginal bacterial infections


Vanić, Željka; Rukavina, Zora; Manner, Suvi; Fallarero, Adyary; Uzelac, Lidija; Kralj, Marijeta; Amidžić Klarić, Daniela; Bogdanov, Anita; Raffai, Tímea; Virok, Dezső Peter et al.
Azithromycin-liposomes as a novel approach for localized therapy of cervicovaginal bacterial infections // International Journal of Nanomedicine, 14 (2019), 5957-5976 doi:10.2147/IJN.S211691 (međunarodna recenzija, članak, znanstveni)


Naslov
Azithromycin-liposomes as a novel approach for localized therapy of cervicovaginal bacterial infections

Autori
Vanić, Željka ; Rukavina, Zora ; Manner, Suvi ; Fallarero, Adyary ; Uzelac, Lidija ; Kralj, Marijeta ; Amidžić Klarić, Daniela ; Bogdanov, Anita ; Raffai, Tímea ; Virok, Dezső Peter ; Jelena Filipović-Grčić, Jelena ; Škalko-Basnet, Nataša

Izvornik
International Journal of Nanomedicine (1178-2013) 14 (2019); 5957-5976

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Vaginal drug delivery ; Biofilm ; Escherichia coli ; Chlamydia trachomatis ; Cervical cells, Biocompatibility

Sažetak
Background: Efficient localized cervicovaginal antibacterial therapy, enabling the delivery of antibiotic to the site of action at lower doses while escaping systemic drug effects and reducing the risk of developing microbial resistance, is attracting considerable attention. Liposomes have been shown to allow sustained drug release into vaginal mucosa and improve delivery of antibiotics to bacterial cells and biofilms. Azithromycin (AZI), a potent broad-spectrum macrolide antibiotic, has not yet been investigated for localized therapy of cervicovaginal infections, although it is administered orally for the treatment of sexually transmitted diseases. Encapsulation of AZI in liposomes could improve its solubility, antibacterial activity, and allow the prolonged drug release in the cervicovaginal tissue, while avoiding systemic side effects. Purpose: The objective of this study was to develop AZI-liposomes and explore their potentials for treating cervicovaginal infections. Methods: AZI-liposomes that differed in bilayer elasticity/rigidity and surface charge were prepared and evaluated under simulated cervicovaginal conditions to yield optimized liposomes, which were assessed for antibacterial activity against several planktonic and biofilm forming Escherichia coli strains and intracellular Chlamydia trachomatis, ex vivo AZI vaginal deposition/penetration, and in vitro cytotoxicity toward cervical cells. Results: Negatively charged liposomes with rigid bilayers (CL-3), propylene glycol liposomes (PGL-2) and deformable propylene glycol liposomes (DPGL-2) were efficient against planktonic E. coli ATCC 700928 and K-12. CL-3 was superior for preventing the formation of E. coli ATCC 700928 and K-12 biofilms, with IC50 values concentrations that inhibit biofilm viability by 50%) up to 8-fold lower than those of the control (free AZI). DPGL-2 was the most promising for eradication of already formed E. coli biofilms and for treating C. trachomatis infections. All AZI-liposomes were biocompatible with cervical cells and improved localization of the drug inside vaginal tissue compared with the control. Conclusion: The performed studies confirm the potentials of AZI-liposomes for localized cervicovaginal therapy.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti



POVEZANOST RADA


Ustanove
Farmaceutsko-biokemijski fakultet, Zagreb,
Institut "Ruđer Bošković", Zagreb

Citiraj ovu publikaciju

Vanić, Željka; Rukavina, Zora; Manner, Suvi; Fallarero, Adyary; Uzelac, Lidija; Kralj, Marijeta; Amidžić Klarić, Daniela; Bogdanov, Anita; Raffai, Tímea; Virok, Dezső Peter et al.
Azithromycin-liposomes as a novel approach for localized therapy of cervicovaginal bacterial infections // International Journal of Nanomedicine, 14 (2019), 5957-5976 doi:10.2147/IJN.S211691 (međunarodna recenzija, članak, znanstveni)
Vanić, Ž., Rukavina, Z., Manner, S., Fallarero, A., Uzelac, L., Kralj, M., Amidžić Klarić, D., Bogdanov, A., Raffai, T. & Virok, D. (2019) Azithromycin-liposomes as a novel approach for localized therapy of cervicovaginal bacterial infections. International Journal of Nanomedicine, 14, 5957-5976 doi:10.2147/IJN.S211691.
@article{article, year = {2019}, pages = {5957-5976}, DOI = {10.2147/IJN.S211691}, keywords = {Vaginal drug delivery, Biofilm, Escherichia coli, Chlamydia trachomatis, Cervical cells, Biocompatibility}, journal = {International Journal of Nanomedicine}, doi = {10.2147/IJN.S211691}, volume = {14}, issn = {1178-2013}, title = {Azithromycin-liposomes as a novel approach for localized therapy of cervicovaginal bacterial infections}, keyword = {Vaginal drug delivery, Biofilm, Escherichia coli, Chlamydia trachomatis, Cervical cells, Biocompatibility} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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