engleski

Comprehensive N-glycosylation analysis of immunoglobulin G from dried blood spots

Immunoglobulin G (IgG) glycans are emerging as a new putative biomarker for biological age and different diseases, requiring a robust workflow for IgG glycome analysis, ideally beginning with a simple and undemanding sampling procedure. Here we report the first comprehensive study on total N-glycans of IgG isolated from dried blood spots (DBS), which was performed in a high- throughput mode. We compared the IgG N-glycan profiles originating from DBS with those originating from plasma, compared different media for DBS collection, evaluated analytical variation and assessed IgG N-glycan profile stability for different storage conditions. In conclusion, we show DBS are a good and stable source material for a robust IgG N-glycan analysis by ultra- performance liquid chromatography (UPLC), suitable for blood sampling in conditions where no trained personnel and necessary lab equipment are available.

IgG glycome ; biomarker ; dried blood spots ; high-throughput ; ultra-performance liquid chromatography

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